(12) Oversettelse av europeisk patentskrift (11) NO/EP 2173868 B1 (19) NO NORGE (51) Int Cl. C12N 9/50 (2006.01) C07K 14/415 (2006.01) C12N 15/29 (2006.01) C12N 15/57 (2006.01) C12N 15/81 (2006.01) A23J 3/34 (2006.01) A61K 38/48 (2006.01) A61Q 19/00 (2006.01) Patentstyret (21) Oversettelse publisert 2013.02.11 (80) Dato for Den Europeiske Patentmyndighets publisering av det meddelte patentet 2012.10.17 (86) Europeisk søknadsnr 08785459.2 (86) Europeisk innleveringsdag 2008.08.08 (87) Den europeiske søknadens Publiseringsdato 2010.04.14 (30) Prioritet 2007.09.14, EP, 07018125 (84) Utpekte stater AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR (73) Innehaver URSAPHARM Arzneimittel GmbH, Postfach 40 01 51, 66057 Saarbrücken, Tyskland (72) Oppfinner MÜLLER, Rolf, Kirkelerstrasse 114, 66440 Blieskastel, Tyskland LUNIAK, Nora, Beim Weisen Stein 8, 66125 Saarbrücken, Tyskland ESCHMANN, Klaus, Lothringer Strasse 26, 66271 Kleinblittersdorf, Tyskland (74) Fullmektig Zacco Norway AS, Postboks 2003 Vika, 0125 OSLO, Norge (54) Benevnelse Rekombinant fremstilling av utvalgte bromelainfraksjoner (56) Anførte publikasjoner WO-A-00/03729, WO-A-00/14253, WO-A-2005/096804 CARTER CAROL E ET AL: "Mutagenesis and kinetic studies of a plant cysteine proteinase with an unusual arrangement of acidic amino acids in and around the active site" BIOCHEMISTRY, vol. 39, no. 36, 12 September 2000 (2000-09-12), pages 11005-11013, XP002472342 ISSN: 0006-2960 CREGG J M ET AL: "RECOMBINANT PROTEIN EXPRESSION IN PICHIA PASTORIS" MOLECULAR BIOTECHNOLOGY, TOTOWA, NJ, US, vol. 16, no. 1, September 2000 (2000-09), pages 23-52, XP001078868 ISSN: 1073-6085 DATABASE EMBL [Online] 14 November 1997 (1997-11-14), "Ananas comosus mrna for ananain precursor, complete cds" XP002472345 retrieved from EBI accession no. EMBL:ACAJ2477 Database accession no. AJ002477 cited in the application DATABASE UniProt [Online] 1 January 1998 (1998-01-01), "FBSB precursor." XP002472346 retrieved from EBI accession no. UNIPROT:O23799 Database accession no. O23799 DATABASE UniProt [Online] 1 January 1998 (1998-01-01), "Fruit bromelain precursor (EC 3.4.22.33) (Allergen Ana c 2)." XP002472347 retrieved from EBI accession no. UNIPROT:O23791 Database accession no. O23791 LUNIAK N: "Heterologe Expression von Proteinen aus Ananas comosus zur Aufklärung der pharmakologischen Wirkmechanismen von Bromelain" 30 October 2007 (2007-10-30), UNIVERSITÄT DES SAARLANDES, SAARBRÜCKEN, XP002472344 the whole document MAURER H R: "Bromelain: biochemistry, pharmacology and medical use" CMLS CELLULAR AND MOLECULAR LIFE SCIENCES, BIRKHAUSER VERLAG, HEIDELBERG, DE, vol. 58, no. 9, August 2001 (2001-08), pages 1234-1245, XP002995539 ISSN: 1420-682X PAWAN GUPTA ET AL: "Bioaffinity Based Oriented Immobilization of Stem Bromelain" BIOTECHNOLOGY LETTERS, KLUWER ACADEMIC PUBLISHERS, DO, vol. 28, no. 12, 8 June 2006 (2006-06-08), pages 917-922, XP019391503 ISSN: 1573-6776