Appendix C. ClustalW alignments and. Blast search results

Transkript

1 Appendix C ClustalW alignments and Blast search results 167

2 NOP56 protein alignment CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Pearson Sequence 1: Arabidopsis-T6H22 Sequence 2: Cicer arietinum Sequence 3: Schizosaccharomyces Sequence 4: Homo sapiens Sequence 5: D.subobscura Sequence 6: Saccharomyces Sequence 7: S acidocaldarius 522 aa 454 aa 497 aa 602 aa 503 aa 504 aa 412 aa Pairwise alignments Sequences (2:3) Aligned. Score: 51 Sequences (1:2) Aligned. Score: 71 Sequences (3:4) Aligned. Score: 51 Sequences (4:5) Aligned. Score: 58 Sequences (2:4) Aligned. Score: 57 Sequences (1:3) Aligned. Score: 55 Sequences (3:5) Aligned. Score: 51 Sequences (4:6) Aligned. Score: 49 Sequences (2:5) Aligned. Score: 52 Sequences (4:7) Aligned. Score: 27 Sequences (1:4) Aligned. Score: 51 Sequences (3:6) Aligned. Score: 58 Sequences (2:6) Aligned. Score: 48 Sequences (5:6) Aligned. Score: 49 Sequences (2:7) Aligned. Score: 29 Sequences (3:7) Aligned. Score: 26 Sequences (1:5) Aligned. Score: 52 Sequences (5:7) Aligned. Score: 27 Sequences (6:7) Aligned. Score: 28 Sequences (1:6) Aligned. Score: 49 Sequences (1:7) Aligned. Score: 32 CLUSTAL W (1.81) multiple sequence alignment Arabidopsis-T6H MAMYVIYESSSGYGLFEVHG-LDEIGQNTEAVRTSVSDLSRFGRVVQLTAFHP 52 Cicer arietinum Schizosaccharomyces -----MA--DYLLYESATGYSLFDVVG-ADQIAAKTKEVQLSLQDISKFGKVVQLRSFIP 52 Saccharomyces -----MAPIEYLLFEEPTGYAVFKVKLQQDDIGSRLKEVQEQINDFGAFTKLIELVSFAP 55 Homo MAGRGAMVLLHVLFEHAVGYALVALKE-VEEISLLQPQVEESVLNLGKFHSIVRLVAFCP 59 D.subobscura MSILYCLYEHAAGFSVFSVKE-FEEVSMFLPQVESSVTDFAKFNSIVKLAGFAP 53 Sulfolobus MKIYLVEHAIGSFG-YDESGKLIDFVP 26 Arabidopsis-T6H22 FESALDALNQVNAVSEGVMTDELRSFLELNLPKVKEGKKPKFSLGLAEPKLGSHIFEATK 112 Cicer arietinum TDELRTVLETNLPKVKEGKKPKFSLGVAESKLGSQIHDVTK 41 Schizosaccharomyces FKNAAHALENANDISEGVLNDFLKNFLELNLPKAS--KKKKVSLGVQDKNLATSIKSEID 110 Saccharomyces FKGAAEALENANDISEGLVSESLKAILDLNLPKASS-KKKNITLAISDKNLGPSIKEEFP 114 Homo FASSQVALENANAVSEGVVHEDLRLLLETHLP-SK---KKKVLLGVGDPKIGAAIQEELG 115 D.subobscura FKTAIAALENINAISEGIVPQDLLHFLDDFFAKLK---KKKCTLGIADAKLGAAITEAIG 110 Sulfolobus NS------KDIGKVTEALIENEKGTPLPSAIELIN--KLKPDQVVVENDAEVPALQKLGI 78 : * :. : : :. :. 168

3 Arabidopsis-T6H22 -IPCQSNEFVLELLRGVRQHFDRFIKDLKPGD-LEKSQLGLAHSYSRAKVKFNVNRVDNM 170 Cicer arietinum -IPCQCNEFVGELLRGVRQHFVDSVGDLKPGD-LIKAQLGLSHSYSRAKVKFNVNRVDNM 99 Schizosaccharomyces AIECDTSELTQDLLRGIRFHGDKLLKQLSPGD-FERAQLGLGHSYSRAKVKFNVNRNDNM 169 Saccharomyces YVDCISNELAQDLIRGVRLHGEKLFKGLQSGD-LERAQLGLGHAYSRAKVKFSVQKNDNH 173 Homo -YNCQTGGVIAEILRGVRLHFHNLVKGLTDLS-ACKAQLGLGHSYSRAKVKFNVNRVDNM 173 D.subobscura -VQCSQVGVVPEILRGVRFHFAKLVKGFTDQS-AGVAQLGLGHSYSRAKVKFNVHRSDNM 168 Sulfolobus KASSEIRNMGTEIFRENLVNVAIQTKIVNSEDELYNFLYELSMEYTRRKLRGAASKRDLL :::* :.. *. *:* *::. : * Arabidopsis-T6H22 VIQAIFMLDTLDKDINSFAMRVREWYSWHFPELVKIVNDNYLYARVSKMIDDKSKLTEDH 230 Cicer arietinum VIQAIFLLDTLDKDINSFSMRVREWYSWHFPELVKIVNDNYLYCKVAKFIEDKSKLSEDK 159 Schizosaccharomyces IIQAIAILDQLDKDINTFAMRMKEWYSWHFPELSKIVGDNYKYAVIVTLVGDKTTINDEM 229 Saccharomyces IIQAIALLDQLDKDINTFAMRVKEWYGWHFPELAKLVPDNYTFAKLVLFIKDKASLNDDS 233 Homo IIQSISLLDQLDKDINTFSMRVREWYGYHFPELVKIINDNATYCRLAQFIGNRRELNEDK 233 D.subobscura IIQSIALLDQLDKDVNTFSMRIREWYSYHFPELVKIVPDNYMFAKAAKFIKDRKNLSQDL 228 Sulfolobus AIQAIRAIDDIDKTINLFSERLREWYSIHFPELDKLIEDHWLYASIISKFGHRDNLSDTG 198 **:* :* :** :* *: *::***. ***** *:: *: :...: :.: Arabidopsis-T6H22 IPMLTEVLGDE-DKAKEVIEAGKASMGSDLSPLDLINVQTFAQKVMDLADYRKKLYDYLV 289 Cicer arietinum LEGLTDQVGDE-DKAKEIIEAAKASMGQDLSPVDLINVHMFAQRVMDLSDYRRRLYDYLT 218 Schizosaccharomyces LHDLAAVVDDDKDIAQSIINAGKVSMGQDISEIDLENILSFAERVIKLSNYRKQLHNYLV 289 Saccharomyces LHDLAALLNEDSGIAQRVIDNARISMGQDISETDMENVCVFAQRVASLADYRRQLYDYLC 293 Homo LEKLEELTMDG-AKAKAILDASRSSMGMDISAIDLINIESFSSRVVSLSEYRQSLHTYLR 292 D.subobscura LEDLEKIVMDS-AKAQAIIDAAKMSMGMDISIVDLMNIELFAERVVKLSEYRKKLSTYLH 287 Sulfolobus LEEIG----LNKEKIEKIMNAAKNSIGADITDVDIKSVKTLSDIILKLYEERLELTDYTE 254 : : : :::.: *:* *:: *:.: ::. :.* : * * * Arabidopsis-T6H22 TKMSDIAPNLAALIGEMVGARLISHAGSLTNLAKCPSSTLQILGAEKALFRALKTRGNTP 349 Cicer arietinum TKMNDIAPNLASLIGEVVGARLISHAGSLTNLAKCPSSTLQILCAEKALFRAFKTRRNTP 278 Schizosaccharomyces QKMNVVAPNLAELIGEMVGARLISHAGSLTNLSKCPASTVQILGAEKALFRALKTRGNTP 349 Saccharomyces EKMHTVAPNLSELIGEVIGARLISHAGSLTNLSKQAASTVQILGAEKALFRALKTKGNTP 353 Homo SKMSQVAPSLSALIGEAVGARLIAHAGSLTNLAKYPASTVQILGAEKALFRALKTRGNTP 352 D.subobscura NKMNLVAPNLQSLIGDQVGARLISHAGSLTNLAKYPASTVQILGAEKALFRALKTRSNTP 347 Sulfolobus SVMREVAPNVTSLVGPTLGARLLSLAGSLEDLAKMPASTIQVLGAEKALFRALRKGGRPP 314 * :**.: *:* :****:: **** :*:*.:**:*:* ********::...* Arabidopsis-T6H22 KYGLIFHSSFIGRASAKNKGRIARYLANKCSIASRIDCFADGATTAFGEKLREQVEERLE 409 Cicer arietinum KYGLIFHSSFIGRASAKNKGRMARYLANKCSIASRIDCFSENGSTIFGEKLREQVEERLD 338 Schizosaccharomyces KYGIIYHSSFIGKAGAKNKGRISRFLANKCSIASRIDNFSDAPTTAFGQVLRRQVEERLN 409 Saccharomyces KYGLIYHSGFISKASAKNKGRISRYLANKCSMASRIDNYSEEPSNVFGSVLKKQVEQRLE 413 Homo KYGLIFHSTFIGRAAAKNKGRISRYLANKCSIASRIDCFSEVPTSVFGEKLREQVEERLS 412 D.subobscura KYGLIYHSSFIGRAGLKNKGRISRFLANKCSIASRIDCFLDQPTSVFGESLKQQVEDRLK 407 Sulfolobus KHGVIFQYPAIHNSPRWQRGKIARALAAKLAIAARVDYFSG---KFIGNKLNEELKKRID 371 *:*:*:: *.: ::*:::* ** * ::*:*:* :. :*. *..:::.*:. Arabidopsis-T6H22 FYDKGVAPRKNVDVMKEVIENLKQEEEGKEPVDASVKKSKK---KKAKGEEEEEVVAMEE 466 Cicer arietinum FYDKGVAPRKNIDVMKSAIEIADNKDTEMETEEASAKKSKK---KKQKATDEDDMAVDNA 395 Schizosaccharomyces FFDTGVAPTRNSIAMAEA---YEKALSSVNIDGDEEVDIDV---EETVETISE-KPSKKE 462 Saccharomyces FYNTGKPTLKNELAIQEAMELYNKDKPAAEVEETKEKESSK---KRKLEDDDEEKKEKKE 470 Homo FYETGEIPRKNLDVMKEAMVQAEAEEAAAEITRKLEKQEKKRLKKEKKRLAALALASSEN 472 D.subobscura FYESGDVPRKNIEVMKEA-IEAAGQEAGANGTAAPQSEKKK--NKKKKRAAGEDETEANG 464 Sulfolobus EIR EKYAQPPPR---KQEQKVEQKGKKERKN 399 :. : Arabidopsis-T6H22 DKSEKKKKKEKRKM ETAEEN-EKSEKKKTKKSKA Cicer arietinum AEVTNGDATEDHKS EKKKKKKEKRKVDLEVETQD Schizosaccharomyces KKDKKEKKKEKSKK KRSADDA-----SEEVKKS Saccharomyces KKSKKEKKEKKEKK DKKEKKD-----KKEKKDK Homo SSSTPEECEETSEKPKKKKKQKPQEVPQENGMEDPSISFSKPKKKKSFSKEELMSSDLEE 532 D.subobscura SSNGNGAAAEAADN GNGNDD-----GEPKKKK Sulfolobus KKDKRRGKKEGRR : 169

4 Arabidopsis-T6H GGEEETDDGHSTKKK 514 Cicer arietinum PSVDEGANGDSSKKK 444 Schizosaccharomyces KKK 493 Saccharomyces KKK 501 Homo TAGSTSIPKRKKSTPKEETVNDPEEAGHRSRSKKKRKFSKEEPVSSGPEEAVGKSSSKKK 592 D.subobscura KKK 494 Sulfolobus Arabidopsis-T6H22 KKKSKSAE Cicer arietinum KKKSKRMDVD 454 Schizosaccharomyces KKSH Saccharomyces SKD Homo KKFHKASQED 602 D.subobscura NKNKEAVQA- 503 Sulfolobus

5 NOP58 protein alignment CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Pearson Sequence 1: Arabidopsis-F108 Sequence 2: Arabidopsis-T12H1 Sequence 3: PeaSARBP1 Sequence 4: PeaSARBP2 Sequence 5: Oryza Sequence 6: Homo Sequence 7: Rattus Sequence 8: D melanogaster Sequence 9: Schizosaccharomyces Sequence 10:Saccharomyces 533 aa 533 aa 560 aa 550 aa 485 aa 529 aa 534 aa 510 aa 508 aa 504 aa Pairwise alignments Sequences (1:2) Aligned. Score: 82 Sequences (4:5) Aligned. Score: 75 Sequences (3:4) Aligned. Score: 86 Sequences (2:3) Aligned. Score: 73 Sequences (1:3) Aligned. Score: 74 Sequences (3:5) Aligned. Score: 77 Sequences (4:6) Aligned. Score: 55 Sequences (2:4) Aligned. Score: 73 Sequences (1:4) Aligned. Score: 73 Sequences (2:5) Aligned. Score: 74 Sequences (3:6) Aligned. Score: 56 Sequences (4:7) Aligned. Score: 55 Sequences (1:5) Aligned. Score: 74 Sequences (2:6) Aligned. Score: 55 Sequences (3:7) Aligned. Score: 55 Sequences (4:8) Aligned. Score: 52 Sequences (1:6) Aligned. Score: 52 Sequences (2:7) Aligned. Score: 55 Sequences (3:8) Aligned. Score: 53 Sequences (4:9) Aligned. Score: 53 Sequences (1:7) Aligned. Score: 54 Sequences (2:8) Aligned. Score: 52 Sequences (3:9) Aligned. Score: 54 Sequences (4:10) Aligned. Score: 40 Sequences (1:8) Aligned. Score: 52 Sequences (2:9) Aligned. Score: 52 Sequences (3:10) Aligned. Score: 41 Sequences (5:6) Aligned. Score: 54 Sequences (1:9) Aligned. Score: 54 Sequences (2:10) Aligned. Score: 40 Sequences (6:7) Aligned. Score: 95 Sequences (5:7) Aligned. Score: 56 Sequences (1:10) Aligned. Score: 41 Sequences (7:8) Aligned. Score: 61 Sequences (6:8) Aligned. Score: 60 Sequences (8:9) Aligned. Score: 50 Sequences (5:8) Aligned. Score: 53 Sequences (7:9) Aligned. Score: 48 Sequences (6:9) Aligned. Score: 49 Sequences (8:10) Aligned. Score: 37 Sequences (5:9) Aligned. Score: 52 Sequences (7:10) Aligned. Score: 37 Sequences (6:10) Aligned. Score: 38 Sequences (9:10) Aligned. Score: 39 Sequences (5:10) Aligned. Score: 39 CLUSTAL W (1.81) multiple sequence alignment Homo ----MLVLFETSVGYAIFKVLNE--KKLQEVDSLWKEFETPEKANKIVKLKHFEKFQDTA 54 Rattus ----MLVLFETSVGYAIFKVLNE--KKLQEVDSLWKEFETPEKANKIVKLKHFEKFQDTA 54 D melanogaster ----MFVLYETPAGYAIFKLLDE--KKLEQVDNLYQEFETPEKANKLLKLKHFEKFNDTT 54 Arabidopsis-F MLILFETPGGFAIFKVLNE--GKLSNVEDLGNEFSTAKLARKMVKLVAFDKFDNTA 54 Arabidopsis-T12H1 ---MVLVLYETAAGFALFKVKDE--GKMANVEDLCKEFDTPDSARKMVKLKAFEKFDNTS

6 PeaSARBP1 ----MLVLFETPAGFALFKVLNE--GKLSEVQDLSLDFSTADAARKVVKLKAFSKFENTA 54 PeaSARBP2 ----MLLLFETAAGFALFKVLDE--GKLSKVEDLQQSFSSADTARKVVKLKAFSKFENTS 54 Oryza ----MLVLFETPAGFALFKVLDE--GKLDKVEDLWKEFTTSDSARKVVELKAFNKFENTS 54 Schizosaccharomyces ----MFILTETAAGYAIFKAKD---KLLKKRDALIEDLKSPEGASNLLKLQSFAKFESTV 53 Saccharomyces MAPIEYLLFEEPTGYAVFKVKLQQDDIGSRLKEVQEQINDFGAFTKLIELVSFAPFKGAA 60 :* *. *:*:**.. :.: ::::* * *..: Homo EALAAFTALMEGKINKQLKKVLKK-IVKEAHE----PLAVADAKLGGVIKEK--LNLSCI 107 Rattus EALAAFTALMEGKINKQLKKVLKK-IVKEAHE----PLAVADAKLGGVIKEK--LNLSCI 107 D melanogaster EALAAATAAVEGKVAKPLKKTLKKLLVDDVQS----SLLVADAKLGTAIKDK--LSVQCV 108 Arabidopsis-F108 EALEAVAKLLEGTPSKGLRKFLKA---NCVGE----TLAVADSKLGNIIKEK--LKIVCV 105 Arabidopsis-T12H1 EALEAVAKLLEGAPSKGLRKFLKA---NCQGE----TLAVADSKLGNVIKEK--LKIDCI 106 PeaSARBP1 EALEAASCLIDGKTSKGLRKFLRA---HCDDE----ILAVADSKLGNIIKEK--LKIDCV 105 PeaSARBP2 EALKSANLLIDGKASKDLRKFLSV---HCQNE----TLGVADTKLASIIKEK--LQIDCV 105 Oryza DALSAATLIIDSKPTKGLRKFLQK---HCEGE----TLAVADSKLGNAIKEK--LKIDCL 105 Schizosaccharomyces DALDNVSALVEGKVSSKLSSLLEG-LSDSKSS----TLVVADPKLGNAINKLPGLEFEII 108 Saccharomyces EALENANDISEGLVSESLKAILDLNLPKASSKKKNITLAISDKNLGPSIKEEF-PYVDCI 119 :** :.. * *.. * ::* :*. *:.. : Homo HSPVVNELMRGIRSQMDGLIPGVEPREMAAMCLGLAHSLSRYRLKFSADKVDTMIVQAIS 167 Rattus HSPVVNELMRGIRSQMDGLIPGVEPREMAAMCLGLAHSLSRYRLKFSADKVDTMIVQAIS 167 D melanogaster CNTGVQELMRCIRQQADSLLGGLPKREMTAMALGLAHSLSRYKLKFSPDKIDTMIVQAQC 168 Arabidopsis-F108 HNNAVMELLRGIRSQLTELISGLGDQDLGPMSLGLSHSLARYKLKFSSDKVDTMIIQAIG 165 Arabidopsis-T12H1 HNNAVMELLRGVRSQFTELISGLGDQDLAPMSLGLSHSLARYKLKFSSDKVDTMIIQAIG 166 PeaSARBP1 HNNAVMELMRGVRYQLTELISGLAVQDMAPMSLGLSHSLSRYKLKFSADKVDTMIVQAIG 165 PeaSARBP2 HNVGVMELMRGVRYQLTELIAGLAVQDMAPMSLGLSHSLSRYKLKFSADKVDTMIVQAIG 165 Oryza HNSAVMELMRRLRNQLTELISGLGAQDLGPMSLGLSHSLSRYKLKFSPEKVDTMIIQAIG 165 Schizosaccharomyces SDSSVQDLYRGIREHLSSLISGLAPSDLNAMSLGLSHSLSRHKLKFSPDKVDTMIVQAIA 168 Saccharomyces SNELAQDLIRGVRLHGEKLFKGLQSGDLERAQLGLGHAYSRAKVKFSVQKNDNHIIQAIA :* * :* : *: *: :: ***.*: :* ::*** :* *. *:** Homo LLDDLDKELNNYIMRCREWYGWHFPELGKIISDNLTYCKCLQKVGDRK---NYASAKLSE 224 Rattus LLDDLDKELNNYIMRCREWYGWHFPELGKIISDNLTYCKCLQKVGDRK---NYASATLSE 224 D melanogaster LLDDLDKELNNYMMRAREWYGWHFPELGKIITDNIAFVKTIKLVGTRD---NMATSDLSD 225 Arabidopsis-F108 LLDDLDKELNTYAMRVREWFGWHFPELAKIVQDNILYAKAVKLMGNRI---NAAKLDFSE 222 Arabidopsis-T12H1 LLDDLDKELNTYAMRVREWYGWHFPELAKIISDNILYAKSVKLMGNRV---NAAKLDFSE 223 PeaSARBP1 LLDDLDKELNTYAMRVREWYGWHFPELTKIIQDNIQYARSVKLMGDRI---NAAKLDFSE 222 PeaSARBP2 LLDDLDKELNTYAMRVREWYGWHFPELTKIILDNIQYAKAVKLMGDRI---NAAKLDFSE 222 Oryza LLDDLDKELNTYAMRVREWYGWHFPELTKIVADNIQYAKVVKMMGDRT---NAESLDFSE 222 Schizosaccharomyces LLDDLDKELNTYAMRVREWYGWHFPEMGKIIQDNLAYARVIKAMGMRT---KCSETDFSD 225 Saccharomyces LLDQLDKDINTFAMRVKEWYGWHFPELAKLVPDNYTFAKLVLFIKDKASLNDDSLHDLAA 239 ***:***::*.: ** :**:******: *:: ** : : : : :. :: Homo LLP--EEVEAEVKAAAEISMGTEVSEEDICNILHLCTQVIEISEYRTQLYEYLQNRMMAI 282 Rattus FLS--EEVEAEVKAAAEISMGTEVSEEDICNILHLCTQVIEISEYRTQLYEYLQNRMMAI 282 D melanogaster ILP--EDVEEKVKEAAEISMGTEISEEDVLNIQCLCDEIISINDYRTHLYDYLKARMMAM 283 Arabidopsis-F108 ILA--DEIEAELKEAAVISMGTEVSDLDLLHIRELCDQVLSLAEYRAQLYDYLKSRMNTI 280 Arabidopsis-T12H1 ILA--DEIEADLKDAAVISMGTEVSDLDLLHIRELCDQVLSLSEYRAQLYDYLKSRMNTI 281 PeaSARBP1 ILT--EEVEAEVKEAAVISMGTEIGELDLSNIRELCDQVLSLSEYRAQLYDYLKSRMNTI 280 PeaSARBP2 ILS--EEVEAEVKEASVISMGTEIVTLDLENIRELCDQVLSLSEYRAQLYDYLKSRMNTI 280 Oryza ILSD-DEVEAQLKEAAVISMGTEVSELDLLNIRELCDQVLALSEYRAQLFDYLRSRMNTI 281 Schizosaccharomyces ILP--EEIEATLKSAAEISMGTEITEEDLDNIVMLADQVLELASYRAQLSEYLRNRMQAI 283 Saccharomyces LLNEDSGIAQRVIDNARISMGQDISETDMENVCVFAQRVASLADYRRQLYDYLCEKMHTV 299 :*. : : : **** :: *: :: :..: :.** :* :** :* :: 172

7 Homo APNVTVMVGELVGARLIAHAGSLLNLAKHAASTVQILGAEKALFRALKSRRDTPKYGLIY 342 Rattus APNVTVMVGELVGARLIAHAGSLLNLAKHAASTVQILGAEKALFRALKSRRDTPKYGLIY 342 D melanogaster APNLTVLVGDTVGARLIAHAGSLINLAKHPSSTVQILGAEKALFRALKTKKDTPKYGLIY 343 Arabidopsis-F108 APNLTALVGELVGARLISHGGSLLNLAKQPGSTVQILGAEKALFRALKTKHATPKYGLIF 340 Arabidopsis-T12H1 APNLTALVGELVGARLISHGGSLLNLSKQPGSTVQILGAEKALFRALKTKHATPKYGLIF 341 PeaSARBP1 APNLTAMVGELVGARLIAHGGSLINLAKQPGSTVQILGAEKALFRALKTKHATPKYGLIY 340 PeaSARBP2 APNLTAMVGELVGARLIAHGGSLLNLAKQPGSTVQILGAEKALFRALKTKHATPKYGLIY 340 Oryza APNLTALVGELVGARLIAHGGSLVNLAKQPGSTIQILGAEKALFRALKTKHATPKYGLIY 341 Schizosaccharomyces APNLTALVGELVGARLIAHAGSLMNLAKQPASTIQILGAEKALFRALKTKHSTPKYGLIY 343 Saccharomyces APNLSELIGEVIGARLISHAGSLTNLSKQAASTVQILGAEKALFRALKTKGNTPKYGLIY 359 ***:: ::*: :*****:*.*** **:*:..**:**************:: *******: Homo HASLVGQTSPKHKGKISRMLAAKTVLAIRYDAFGEDSSSA--MGVENRAKLEARLRTLED 400 Rattus HASLVGQTSPKHKGKISRMLAAKTVLAIRYDAFGEDSSSA--MGAENRAKLEARLRILED 400 D melanogaster HAQLVGQASQKNKGKMSRSLAAKASLATRVDAFGEEATFE--LGAAHKVKLESRLRLLEE 401 Arabidopsis-F108 HASVVGQAAPKNKGKISRSLAAKSVLAIRCDALGDSQDNT--MGVENRLKLEARLRTLEG 398 Arabidopsis-T12H1 HASLVGQAAPKHKGKISRSLAAKTVLAIRVDALGDSQDNT--MGLENRAKLEARLRNLEG 399 PeaSARBP1 HASLIGQAAPKFKGKISRSLAAKTALAIRCDALGDGQDNT--MGLENRAKLEARLRNLEG 398 PeaSARBP2 HASLIGQAAPKFKGKISRSLAAKTALAIRCDALGDGQDNT--MGLENRAKLEARLRGLEG 398 Oryza HASLIGQAAPKHKGKISRSLAAKTALAIRYDALGDGEDNS--IGLESRVKLETRLRVLEG 399 Schizosaccharomyces HASLVGQANSKNKGKIARVLATKAALSLRVDALSDKDTTNGNIGLENRIRVENRLRSLEG 403 Saccharomyces HSGFISKASAKNKGRISRYLANKCSMASRIDNYSEEPSNV--FGSVLKKQVEQRLEFYNT 417 *:.:.:: * **:::* ** * :: * *.: :* : ::* **. : Homo RGIRKISGTGKALAKTEKYEHK SEVKTYDPSGDSTLPTCSKKRKIEQVDKE 451 Rattus RGIRKISGTGKALAKAEKYEHK SEVKTYDPSGDSTLPTCSKKRKIEEVDKE 451 D melanogaster GNLRKLSGTGKAKAKFEKYQAK SEVFTYQPEADNTLN--VKKRKHSESEQQ 450 Arabidopsis-F108 KDLGRLSGSAKGKPKIEVYDKDKKKGSGGLITPAKTYNTAADSLLQTPTVDS--ENGVKE 456 Arabidopsis-T12H1 KDLGRLSGSSKGKPKIEVYNKDKKMGSGGLITPAKTYNTAADSLLGETSAKS--EEPSK- 456 PeaSARBP1 KELGRFAGSAKGKPKIEAYDKDKKKGAGGLITPAKTYNTAADSVIHQKSNSAMDEDTPEP 458 PeaSARBP2 KELGHFAGSAKGKPKIEAYDKDKKKGSGGLITPAKTYNTAADSVIEPKSNSAMDEDTPET 458 Oryza KELGRSAGSTKGKPKIEVYEKDRKKGAGALITPAKTYNPAADLVLGQST-----EETPK- 453 Schizosaccharomyces GKLLPLPTAPVQQSKVQINGTS AYSTATDAVTKDAEESQ--EDVEMD 448 Saccharomyces GKP-TLKNELAIQEAMELYNKD KPAAEVEETKEKESSKKRKLEDDDEE 464 :.. :.: Homo DEITEKKAKKAKI KVKVEEEEEEKVAEEE------E 481 Rattus DEITEKKAKKAKI KIKAEVEEEMEEAEEEQVVE-EE 486 D melanogaster TPVKKEEPAEEEA EVKSE Arabidopsis-F108 KKDKKKKKKADD EEEAKTEEPSKKKSNKKKT---E 488 Arabidopsis-T12H1 KKDKKKKKKVE EEKPEEEEPSEKK-KKKKA---E 486 PeaSARBP1 TTAEKKKEKKEKKEKKDKKEKKKKEEVSATLLADVDGVEEPEHEVVKKEKKKRKQSS--E 516 PeaSARBP2 PVTGKKKEKKEKK-KREKKEEK VEDVVEEPEQEVVKKEKKKKKKESTEK 506 Oryza KPE Schizosaccharomyces IVIEKKKKKSSK LKEADG Saccharomyces Homo TSVKKKKKRGKKKHIKEEPLSEEEPCTSTAIASPEKKKKKKKKRENED- 529 Rattus PTVKKKKKKDKKKHIKEEPLSEEEPCTSTAVPSPEKKKKKKKKKDAED- 534 D melanogaster ---KKKKKKKKQKDEEVEAVEEAAAPEPEDQPTPAKKKKKSKHQE Arabidopsis-F108 AEPETAEEPAKKEKKK---KRKHEEEE-TEMPAKKKEKSEKKKKKKTEV 533 Arabidopsis-T12H1 AETEAVVEVAKEEKKKNKKKRKHEEEETTETPAKKKDKKEKKKKSKD PeaSARBP1 NETQNGDTNLNEGEKKKKRKKHAEQEDSADVPSKKNKKKKKNDD PeaSARBP2 AEVQNGDNNLNGGEKKKKRKKSAEEEDTTDMPSKKKEKKKKKDV Oryza GASKKRKHQEAEPAGAEETIQEDGDQEGQ Schizosaccharomyces ---ESSKKEKKEKKDKKHKKSKRKSEESEDGESPKKKKKSKKSKD Saccharomyces ---KKEKKEKKSKKEKKEKKEKKDKKEKKDKKEKKDKKKKSKD :.:.. 173

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