SynNanoVect lipofection 2019

Transkript

1 SynNanoVect lipofection /12

2 Lipofection Amphiphiles& Transfection Nucleic acid Ctrl- Ctrl+ Test Cationic lipid Liposomes lipoplexes Transfection in vitro or in vivo Simple protocol to transfect a wide variety of cells High transfectionefficacies and low toxicity 2/12

3 New tools SNV-1 SNV-1 ptimized for the transfection of HepaRG cell line TransfectionofprogenitorHepaRGatday1 % of transfected cells 64 % 74 % Intensity of fluorescence Side-effects MTT assay 40 % 3/12 SNV-1 SNV-1 Comparison of transfection efficacies and toxicity with two other commercial kits SNV-1

4 New tools SNV-2 SNV-2 ptimizedfor transfectionofhela, A549, 16HBE, SKMel28, C2C12 Efficacy Intensity of bioluminescence Side-effects MTT assay (% cell viability) 4/12

5 Electroporation Dual electroporation systems For cells hard to transfect by lipofection MICRPRATR Neon system Cells in suspension Invitrogen-Life Sciences NUCLEFECTR 4D Cells in suspension and adhrent cells NB: CoreUnit, X Unit (cuvettes), Y Unit (24 well-platew) & Shuttle modulepour for 96 well-plates AMAXA/LNZA 5/12

6 In vivo applications Bioluminescence imaging Biofluorescence imaging 1 6/12

7 PRICE SNV-1 ptimized for the transfection of HepaRG cell line 1 ml ( 100 transfections-2.5x10 5 cellseachtime) 562 euros HT Contact : Tristan.Montier@univ-brest.fr SNV-2 1 ptimizedfor the transfectionofhela, A549, 16HBE, SKMel28, C2C12 1 ml ( 100 transfections-2.5x10 5 cellseachtime) 562 euros HT Contact : Tristan.Montier@univ-brest.fr 7/12

8 SynNanoVect Trimannosylatedlipid as a targetinglipid /12

9 Dendritic cell targeting lipid Tri-Man-Lip100 for trimannosylated liposomes, lipoplexes H H H (LR) and lipopolyplexes(lpr) H H H H H H H H H H N 5 N H Tri-Man-Lip100 9/12

10 Dendritic cell targeting lipid Liposomes, Lipoplexesand lipopolyplexesincorporating Tri-Man-Lip100 induce strong binding on DC cells mediated by the mannose receptor Carbohydr. Res., 2016, 435, Lipopolyplexeswith Tri-Man-Lip100 give rise to an efficient stimulatory immune response allowing anti-cancer vaccination in mice J. Control. Release, 2018, 278, Lipopolyplexeswith Tri-Man-Lip100 combine strong antitumort Cell immunity with improved inflammatory safety ACS Nano, 2018, 12, /12

11 PRICE Tri-Man-Lip100 1 ptimized for the targeting of dendritic cells 1 mg 678,5 euros HT Contact : Tristan.Montier@univ-brest.fr 11/12

12 Transfection& Research SynNanoVect Platform Services, expertise and tools for transfection or vectorisation 4 research groups from Brittany (Brest & Rennes) Member of the Biogenouest platform network Awarded IBISA since 2008 IS 9001 since 2013 ur publications in the field of lipofection since 2010 (selection) J. Med. Chem. 2010, 53, ; Biotechnology J., 2010, 5, ; rg. Biomol. Chem., 2011, 9, ; J. Drug Targeting, 2011, 19, ; Nanomedicine: NBM 2011, 7, ; Eur. J. rg. Chem., 2011, ; Biochimie, 2012, 94, ; Int. J. Pharm.., 2012, 423, ; Biomaterials, 2012, 33, ; rg. Biomol. Chem., 2012, 10, ; Biomaterials, 2012, 33, ; Biophys. Chem., 2013, 171, ; Adv. Healthcare Mater, 2013, 2, ; rg. Biomol. Chem., 2013, 11, ; Eur. J. rg. Chem., 2014, ; Int. J. Pharm., 2014, 460, 1-2, ; Methods Mol. Biol., 2013, 987, ; rg. Biomol. Chem., 2014, 12, ; Mol. Pharm., 2014, 11, ; Eur. J. rg. Chem., 2014, ; rg. Biomol. Chem., 2015, 13, ; Mol. Pharm., 2015, 12, ; Biotechnol. J., 2015, 10, ; Colloids and Surfaces B: Biointerfaces, 2015, 136, ; Nanomedicine: NBM, 2015, 11, ; Biomaterials, 2015, 59, 1-11 ; Inter. J. Mol. Sci., 2015, 16, ; J. Gene Med, 2016, 18, 3-15 ; rg. Biomol. Chem., 2016, 14, ; rg. Biomol. Chem., 2016, 14, ; Soft Matter, 2016, 12, ; Int. J. Pharm.., 2017, 524, ; J. Mater. Chem. B, 2017, 5, ; Inter. J. Pharm., 2018, 536, ; J. Control. Release, 2018, 278, ; ACS Nano, 2018, 12, ; 12/12