Accession start end. Bifunctional purine biosynthesis protein ADE17 [Includes: Phosphoribosylaminoimidazolecarboxamide formyltransferase

Transkript

1 Table S1A. List of 379 unique N-terminal peptides (start position 1 or 2) identified in the proteome of the control yeast strain ( 12 C6 ), the ynata deficient strain expressing hnata ( 13 C6) and/or the ynata deficient strain ( 13 C6 15 N4 L-arginine). UniProt database primary accession number, start and end positions, corresponding peptide sequence identified with initiator Met attached, N-terminal sequence (P1 -P5 ), peptide sequence, modification status/states confirmed by MS/MS, Uniprot entry name, overall α-n-modification status, protein description, number of identified spectra (spectra #), highest Mascot ion score, threshold and, whenever a peptide matched to multiple members of a protein family (redundancy), isoforms are given from all identified unique yeast N-terminal peptides. Accession start end ID initiator Met Score of highest Identity attached Acetylation Free P1'-P5' Sequence Modification_N-term (MS/MS) Overall UNIPROT Description isoforms Spectra # scoring spectra threshold P AAEKI AAEKILTPESQLKKSKAQQKTAEQVAAER prop / Ace / AcD3 ynata/hnata (partial Ac) RL7A_YEAST 60S ribosomal protein L7-A Q no AAGVP AAGVPKIDALESLGNPLEDAKR AcD3 100% free RPIA_YEAST Ribose-5-phosphate isomerase P no AAQKS AAQKSFR prop / AcD3 100% free RL39_YEAST 60S ribosomal protein L P AASVP AASVPPGGQR Ace / AcD3 ynata/hnata (partial Ac) SC6B2_YEAST Protein transport protein SEB P AATKE AATKEAKQPKEPKKR prop / Ace / AcD3 ynata/hnata (partial Ac) NHP6B_YEAST Non-histone chromosomal protein 6B Q ? ADLQK ADLQKQENSSR Ace ynata/hnata NUP53_YEAST Nucleoporin NUP P ADYSS ADYSSLTVVQLKDLLTKR AcD3 ynata/hnata (partial Ac) THO1_YEAST Protein THO P AEASI AEASIEKTQILQKYLELDQR prop / Ace / AcD3 ynata/hnata (partial Ac) GLNA_YEAST Glutamine synthetase Q ? AEEST AEESTDFSQFEEER Ace ynata/hnata GDIR_YEAST Rho GDP-dissociation inhibitor P (*)? AEKEE AEKEEAIFR Ace ynata/hnata VPH1_YEAST Vacuolar ATP synthase subunit a, vacuolar isoform P AFLNI AFLNIFKQKR Ace / AcD3 ynata/hnata (partial Ac) APD1_YEAST Actin patches distal protein P AFQKD AFQKDAKSSAYSSR prop / Ace / AcD3 <5% Ac (ynata/hnata)* RL5_YEAST 60S ribosomal protein L P AGAIE AGAIENAR prop / Ace / AcD3 ynata/hnata (partial Ac) VATA_YEAST Vacuolar ATP synthase catalytic subunit A Q AGENP AGENPKKEGVDAR Ace / AcD3 ynata/hnata (partial Ac) ESF1_YEAST Pre-rRNA-processing protein ESF P AGKKI AGKKIAGVLGATGSVGQR prop / Ace / AcD3 ynata/hnata (partial Ac) DHAS_YEAST Aspartate-semialdehyde dehydrogenase P0C2H no AGLKD AGLKDVVTR prop / AcD3 100% free RL31A_YEAST 60S ribosomal protein L31-A P0C2H9 (2-10) Q AGNAN AGNANSVDEEVTR AcD3 ynata/hnata (partial Ac) PVH1_YEAST Tyrosine-protein phosphatase YVH P no AGQVL AGQVLDGKACAQQFR AcD3 100% free C1TC_YEAST C-1-tetrahydrofolate synthase, cytoplasmic Q AGSQL AGSQLKNLKAALKAR AcD3 ynata/hnata (partial Ac) NOP14_YEAST Nucleolar complex protein P no AGVKA AGVKAYELR prop / AcD3 100% free RL35_YEAST 60S ribosomal protein L P ? AGVSV AGVSVR AcD3 free- RS19B_YEAST 40S ribosomal protein S19-B (S16a) (RP55B) (YS16B) P no AIKSL AIKSLESFLFER AcD3 100% free MKT1_YEAST Protein MKT P no AKEST AKESTGFKPGSAKKGATLFKTR AcD3 100% free CYC7_YEAST Cytochrome c iso P0C2H no AKFLK AKFLKAGKVAVVVR AcD3 100% free RL27A_YEAST 60S ribosomal protein L27-A P0C2H7 (2-15) Q no AKKKS AKKKSKSR AcD3 100% free UTP14_YEAST U3 small nucleolar RNA-associated protein P no AKKVA AKKVAICTLLYSR AcD3 100% free GLG2_YEAST Glycogen synthesis initiator protein GLG P no AKQSL AKQSLDVSSDR prop / AcD3 100% free RL26A_YEAST 60S ribosomal protein L26-A P53221 (2-12) P no AKTIK AKTIKVIR AcD3 100% free YG1I _YEAST Uncharacterized membrane protein YGR026W P no ALGNE ALGNEINITNKLKR AcD3 100% free RPF1_YEAST Ribosome production factor P ? AMNTG AMNTGGFDSMQR Ace ynata/hnata MON2_YEAST Protein MON P ? ANDLV ANDLVQAR Ace ynata/hnata RS14B_YEAST 40S ribosomal protein S14-B P no ANYTK ANYTKTAILSVYDKTGLLDLAR AcD3 100% free PUR92 _ YEAST Bifunctional purine biosynthesis protein ADE17 [Includes: Phosphoribosylaminoimidazolecarboxamide formyltransferase P X no APGKK APGKKVAPAPFGAKSTKSNKTR prop / AcD3 100% free RL8A_YEAST 60S ribosomal protein L8-A Q no APLTK APLTKKTNGKR prop / AcD3 100% free PUF6_YEAST Protein PUF P no APSAK APSAKATAAKKAVVKGTNGKKALKVR prop / AcD3 100% free RL25_YEAST 60S ribosomal protein L P ? AQFSD AQFSDIDKLAVSTLR prop free- TKT2_YEAST Transketolase P ASEKE ASEKEIR prop / AcD3 ynata/hnata (partial Ac) FPPS_YEAST Farnesyl pyrophosphate synthetase UBA P (*) ASIGS ASIGSQVR Ace / AcD3 ynata/hnata (partial Ac) GCN20_YEAST Protein GCN20 VPH P ASNEV ASNEVLVLR prop / Ace / AcD3 ynata/hnata (partial Ac) GBLP_YEAST Guanine nucleotide-binding protein subunit beta-like protein P ? ASTSN ASTSNTFPPSQSNSSNNLPTSR Ace- Ac- YB64_YEAST Uncharacterized protein YBR214W Q ? ASVLL ASVLLPQLR AcD3 free- DIB1_YEAST Spliceosomal protein DIB Q ? ATFNP ATFNPQNEMENQAR Ace ynata/hnata YD173_YEAST Uncharacterized protein YDL173W P ATIAP ATIAPTAPITPPMESTGDR prop / Ace / AcD3 ynata/hnata (partial Ac) Protein URA1 [Includes: Glutamine-dependent carbamoyl- PYR1 _ YEAST phosphate synthase P ? ATKQL ATKQLLR Ace ynata/hnata FTHC_YEAST 5-formyltetrahydrofolate cyclo-ligase P ATLEE ATLEELDAQTLPGDDELDQEILNLSTQELQTR AcD3 ynata/hnata (partial Ac) PRS6A_YEAST 26S protease regulatory subunit 6A Q ? ATMET ATMETTTQKDTNILKSGLKKTIGVLNEAVLQNG R Ace- Ac- NBP2_YEAST NAP1-binding protein Q ? ATPSN ATPSNKR prop free- HAS1_YEAST ATP-dependent RNA helicase HAS P ? ATPST ATPSTGANNSIPAVR Ace ynata/hnata TEM1_YEAST Protein TEM P no AVGKN AVGKNKR prop / AcD3 100% free RS3B_YEAST 40S ribosomal protein S1-B P33442 (2-8) P ? AVIKK AVIKKGAR prop free- MYO3_YEAST Myosin P no AVKGL AVKGLGKPDQVYDGSKIR prop / AcD3 100% free RIB4_YEAST 6,7-dimethyl-8-ribityllumazine synthase O AVKTG AVKTGIAIGLNKGKKVTQMTPAPKISYKKGAAS NR AcD3 ynata/hnata (partial Ac) RL36B_YEAST 60S ribosomal protein L36-B P no AVSKV AVSKVYAR prop / AcD3 100% free ENO1_YEAST Enolase 1 P00925 (2-9) Q3E ? AVSNN AVSNNNNNNNSKER prop free- YL361_YEAST Uncharacterized protein YLR361C-A P no AYADS AYADSPENAIAVIKQR AcD3 100% free VPS35_YEAST Vacuolar protein sorting-associated protein P no CGIFA CGIFAAFR prop / AcD3 100% free ASNS1_YEAST Asparagine synthetase [glutamine-hydrolyzing] Q X?? DPELQ DPELQAIR NH2-? YMW4_YEAST Uncharacterized protein YMR074C P X?? EFVAE EFVAER NH2-? KAPB_YEAST camp-dependent protein kinase type P X no GAYKY GAYKYLEELQR prop / AcD3 100% free RL15A_YEAST 60S ribosomal protein L15-A P GGILK GGILKNPLALSPEQLAQQDPETLEEFR AcD3 ynata/hnata (partial Ac) GLC8_YEAST Protein GLC P no GIIDK GIIDKIKAIEEEMAR AcD3 100% free YG3Y_YEAST Uncharacterized GTP-binding protein YGR173W P ? GINNP GINNPIPR prop free- YSC84_YEAST Protein YSC84 P43603 (2-9) P no '/ GIPKF GIPKFFR AcD3 100% free XRN1_YEAST 5'-3' exoribonuclease Q no GKAKK GKAKKTR AcD3 100% free FCF1_YEAST rrna-processing protein FCF P no GKGKP GKGKPR AcD3 100% free RS23_YEAST 40S ribosomal protein S

2 P no GKGTP GKGTPSFGKR prop / AcD3 100% free RL37A_YEAST 60S ribosomal protein L37-A P51402 (2-11) P no GKKNT GKKNTKGGKKGR prop / AcD3 100% fre IF1A_YEAST Eukaryotic translation initiation factor 1A P no GKNVL GKNVLLLGSGFVAQPVIDTLAANDDINVTVACR AcD3 100% free LYS9_YEAST Saccharopine dehydrogenase [NADP+, L-glutamate-forming] P no GLFDK GLFDKVKQFANSNNNNNDSGNNNQGDYVTKA ENMIGEDR AcD3 100% free DDR48_YEAST Stress protein DDR Q no GSEED GSEEDKKLTKKQLKAQQFR prop / AcD3 100% free NOP6_YEAST Nucleolar protein Q no GTEDA GTEDAIALPNSTLEPR AcD3 100% free HSD1_YEAST Protein HSD P X no GVEQI GVEQILKR prop / NH2- / AcD3 100% free ALF_YEAST Fructose-bisphosphate aldolase P no IIEPS IIEPSLKALASKYNCDKSVCR AcD3 100% free RL40_YEAST 60S ribosomal protein L Q ? LMPKE LMPKEDR prop free- RS10A_YEAST 40S ribosomal protein S10-A P no MAIKK MAIKKR AcD3 100% free NTR2_YEAST Pre-mRNA-splicing factor NTR P X? MAPGK MAPGKKVAPAPFGAKSTKSNKTR Ace- ynatb/c RL8A_YEAST 60S ribosomal protein L8-A P ? MDAGL MDAGLSTMATR Ace- ynatb/c YM11_YEAST Uncharacterized protein YMR124W P ? MDAYS MDAYSTR Ace- ynatb/c FAS1_YEAST Fatty acid synthase subunit beta P ? MDEVL MDEVLPLFR Ace- ynatb/c COG1_YEAST Conserved oligomeric Golgi complex subunit P ? MDFET MDFETNEDINGVR Ace- ynatb/c SEC23_YEAST Protein transport protein SEC P ? MDGMF MDGMFAMPGAAAGAASPQQPKSR Ace- ynatb/c TOM6_YEAST Mitochondrial import receptor subunit TOM P ? MDIIL MDIILGIR Ace- ynatb/c PSB2_YEAST Proteasome component C P ? MDKKK MDKKKDLLENEQFLR Ace- ynatb/c ARE2_YEAST Sterol O-acyltransferase P ? MDKNS MDKNSVNKDSEEKDER Ace- ynatb/c SDS22_YEAST Protein phosphatase 1 regulatory subunit SDS Q ? MDNGT MDNGTDSSTSKFVPEYR Ace- ynatb/ IMA1_YEAST Importin subunit alpha Q3E7X9 1 18? MDNKT MDNKTPVTLAKVIKVLGR Ace- ynatb/c RS28A_YEAST 40S ribosomal protein S28-A P ? MDNQQ MDNQQESISEDITGDLAAAVR Ace- Ac- IDS2_YEAST IME2-dependent-signaling protein Q X? MDPEL MDPELQAIR Ace- ynatb/c YMW4_YEAST Uncharacterized protein YMR074C Q ? MDPFL MDPFLEFR Ace- ynatb/c RAD28_YEAST Radiation-sensitive protein Q ? MDQSV MDQSVEDLFGALR Ace- ynatb/c FCF2_YEAST rrna-processing protein FCF P ? MDSEV MDSEVAALVIDNGSGMCKAGFAGDDAPR Ace- ynatb/c ACT_YEAST Actin P0C0X0 1 18? MDSKT MDSKTPVTLAKVIKVLGR Ace- ynatb/c RS28B_YEAST 40S ribosomal protein S28-B P ? MDSQP MDSQPVDVDNIIDR Ace- ynatb/c PP12_YEAST Serine!threonine-protein phosphatase PP Q ? MDSQV MDSQVPNNNESLNR Ace- ynatb/c TBF1_YEAST Protein TBF P ? MDTLF MDTLFNSTEKNAR Ace- Ac- PFD3_YEAST Prefoldin subunit Q ? MDTVG MDTVGTDAAAASINER Ace- ynatb/c YDR86_YEAST Uncharacterized protein YDR186C P ? MDTVN MDTVNNYR Ace- ynatb/c IPMK_YEAST Inositol polyphosphate multikinase P X? MEFVA MEFVAER Ace- ynatb/c KAPB_YEAST camp-dependent protein kinase type P ? MELLP MELLPQGQR Ace- ynatb/c PFD4_YEAST Prefoldin subunit P ? MELSP MELSPTYQTER Ace- ynatb/c NUP84_YEAST Nucleoporin NUP P0C0V ? MENDK MENDKGQLVELYVPR Ace- ynatb/c RS21A_YEAST 40S ribosomal protein S21-A Q3E754 (1-15) P ? MENIT MENITQPTQQSTQATQR Ace- ynatb/c RAD53_YEAST Serine!threonine-protein kinase RAD Q ? MEQNA MEQNAEKR Ace- ynatb/c DUS3_YEAST trna-dihydrouridine synthase P ? METID METIDIQNRSFVVR Ace- ynatb/c OSH3_YEAST Oxysterol-binding protein homolog P ? METPL METPLDLLKLNLDER Ace- ynatb/c LSM3_YEAST U6 snrna-associated Sm-like protein LSm P no MFGLP MFGLPQQEVSEEEKR AcD3 100% free TOM5_YEAST Mitochondrial import receptor subunit TOM P ? MFKKF MFKKFTR Ace- ynatb/c TMA20_YEAST Translation machinery-associated protein P X?? MGAYK MGAYKYLEELQR NH2-? RL15A_YEAST 60S ribosomal protein L15-A P X no MGVEQ MGVEQILKR prop / AcD3 100% free ALF_YEAST Fructose-bisphosphate aldolase P ? MIAPD MIAPDSQR Ace- Ac- SMY2_YEAST Protein SMY P no MIKST MIKSTLIYR AcD3 100% free SEC22_YEAST Protein transport protein SEC P no MIPAP MIPAPLDASLLR AcD3 100% free YCT3_YEAST Uncharacterized protein YCR043C P MIVPT MIVPTYGDVLDASNR prop / Ace- (partial Ac) SRY1_YEAST Threo-3-hydroxyaspartate ammonia-lyase P no MKEVV MKEVVVSETPKR AcD3 100% free RPC1_YEAST DNA-directed RNA polymerase III subunit RPC P no MKGLI MKGLILVGGYGTR prop / AcD3 100% free MPG1_YEAST Mannose-1-phosphate guanyltransferase P no MKLDI MKLDIKKTFSNR AcD3 100% free COPB2_YEAST Coatomer subunit beta' P no MKMLT MKMLTKFESKSTR AcD3 100% free COPA_YEAST Coatomer subunit alpha P no MKSDF MKSDFKFSNLLGTVYR AcD3 100% free PWP2_YEAST Periodic tryptophan protein Q3E no MKSSI MKSSIPITEVLPR prop / AcD3 100% free YN034_YEAST Uncharacterized protein YNR034W-A P no MKVEI MKVEIDSFSGAKIYPGR AcD3 100% free RL24A_YEAST 60S ribosomal protein L24-A P ? MKVEV MKVEVDSFSGAKIYPGR prop free- RL24B_YEAST 60S ribosomal protein L24-B Q no MKVGG MKVGGIEDR AcD3 100% free CDD_YEAST Cytidine deaminase Q no MKVGI MKVGIIMGSVR AcD3 100% free LOT6_YEAST NAD(P)H-dependent FMN reductase LOT P no MKVLE MKVLEER AcD3 100% free RPC9_YEAST DNA-directed RNA polymerase III subunit RPC P ? MLAAK MLAAKNILNR Ace- ynatb/c HSP77_YEAST Heat shock protein SSC1, mitochondrial precursor P no MLAEK MLAEKTR prop / AcD3 100% free FHP_YEAST Flavohemoprotein P ? MLCAI MLCAISGKVPR Ace- ynatb/c PRP19_YEAST Pre-mRNA-splicing factor P no MLDIN MLDINQFIEDKGGNPELIR AcD3 100% free SYSC_YEAST Seryl-tRNA synthetase, cytoplasmic P no MLFDN MLFDNKNR prop / AcD3 100% free DHE2_YEAST NAD-specific glutamate dehydrogenase Q X no MLMPK MLMPKEDR prop / AcD3 100% free RS10A_YEAST 40S ribosomal protein S10-A P no MLMPK MLMPKQER AcD3 100% free RS10B_YEAST 40S ribosomal protein S10-B P ? MLNFT MLNFTGQTR Ace- ynatb/c HUL5_YEAST Probable E3 ubiquitin-protein ligase HUL P ? MLVFG MLVFGPNSSFVR Ace- ynatb/c MSN4_YEAST Zinc finger protein MSN P no MLYEL MLYELIGLVR AcD3 100% free RT06_YEAST Mitochondrial 37S ribosomal protein MRP P X? MMNNN MMNNNGNQVSNLSNALR Ace- ynatb/c URE2_YEAST Protein URE P ? MMSNV MMSNVANASQR Ace- ynatb/c NGR1_YEAST Negative growth regulatory protein NGR P ? MNDQT MNDQTQFTER Ace- ynatb/c HS104_YEAST Heat shock protein Q ? MNEVP MNEVPTTPVR Ace- ynatb/c SLK19_YEAST Kinetochore protein SLK P ? MNGIQ MNGIQVDINR Ace- ynatb/c PSB6_YEAST Proteasome component PRE3 precursor

3 P ? MNGKE MNGKEVSSGSGR Ace- ynatb/c AVT3_YEAST Vacuolar amino acid transporter P ? MNISG MNISGTLNTLR Ace- ynatb/c YHQ0_YEAST Uncharacterized protein YHR100C P MNIVK MNIVKLQR prop / Ace- ynatb/c (partial Ac) FIMB_YEAST Fimbrin P ? MNKEE MNKEELLGFLLDDSIDSQKR Ace- ynatb/c YKC3_YEAST Uncharacterized protein YKL023W P ? MNLLE MNLLETR Ace- ynatb/c PRP9_YEAST Pre-mRNA-splicing factor PRP P X? MNNNG MNNNGNQVSNLSNALR Ace- ynatb/c URE2_YEAST Protein URE Q ? MNNNN MNNNNVTEATSR Ace- ynatb/c UGO1_YEAST Protein UGO P ? MNNNV MNNNVEELLR Ace- ynatb/c YG4G_YEAST Uncharacterized protein YGR206W Q ? MNNSE MNNSEDPFQQVVKDTKEQLNR Ace- ynatb/c TLG1_YEAST T-SNARE affecting a late Golgi compartment protein P ? MNNSI MNNSIITDDEVR Ace- ynatb/c YGP9_YEAST Uncharacterized protein YGL159W P ? MNQEE MNQEEAIFR Ace- ynatb/c STV1_YEAST Vacuolar ATP synthase subunit a, Golgi isoform P ? MNQIL MNQILNAQR Ace- ynatb/c YJ81_YEAST Uncharacterized protein YJR111C P ? MNQLG MNQLGALAQVSR Ace- ynatb/c PRA1_YEAST Prenylated Rab acceptor P ? MNSLV MNSLVTQYAAPLFER Ace- ynatb/c ELO2_YEAST Elongation of fatty acids protein P ? MNTEG MNTEGASLSEQLLDAAR Ace- ynatb/c YGZ2_YEAST Ankyrin repeat-containing protein YGL242C P ? MNTNV MNTNVPIFSSPVR Ace- ynatb/c GAL3_YEAST Protein GAL P ? MNVST MNVSTSTFQTR Ace- ynatb/c RPB4_YEAST DNA-directed RNA polymerase II subunit RPB P ? MNVTT MNVTTPEVAFR Ace- ynatb/c ORC5_YEAST Origin recognition complex subunit P X no MPKLV MPKLVLVR AcD3 100% free PMG1_YEAST Phosphoglycerate mutase P ? MQAPV MQAPVVFMNASQER Ace- ynatb/c TCPG_YEAST T-complex protein 1 subunit gamma P ? MQAQG MQAQGSQSNVGSLR Ace- Ac- YBP3_YEAST Uncharacterized transporter YBR043C Q ? MQKTD MQKTDGIR Ace- ynatb/c SGD1_YEAST Suppressor of glycerol defect protein P ? MQQDD MQQDDDFQNFVATLESFKDLKSGISGSR Ace- ynatb/c NRD1_YEAST Protein NRD P ? MQSMN MQSMNVQPR Ace- ynatb/c POP2_YEAST Protein POP P MQSQD MQSQDSCYGVAFR Ace- / AcD3 ynatb/c (partial Ac) K6PF1_YEAST 6-phosphofructokinase subunit alpha Q no MQVKS MQVKSIKMR prop / AcD3 100% free GLO2_YEAST Hydroxyacylglutathione hydrolase, cytoplasmic isozyme P X no MSEIT MSEITLGKYLFER AcD3 100% free PDC1_YEAST Pyruvate decarboxylase isozyme 1 P16467 (1-13)^AP26263 (1-13) P ? MTEFK MTEFKAGSAKKGATLFKTR prop free- CYC1_YEAST Cytochrome c iso P no MTPEA MTPEAKKR prop / AcD3 100% free SEC16_YEAST COPII coat assembly protein SEC P X MVAFT MVAFTVDQMR Ace- / AcD3 ynatb/c (partial Ac) EF2_YEAST Elongation factor P ? MYSTP MYSTPLKKR Ace- ynatb/c NU157_YEAST Nucleoporin NUP Q no PALLK PALLKR prop / AcD3 100% free YL301_YEAST Uncharacterized protein YLR301W P ? PAPQD PAPQDPR prop free- HEM6_YEAST Coproporphyrinogen III oxidase Q ? PELTE PELTEFQKKR prop free- YD156_YEAST WD repeat-containing protein YDL156W P no PEQEP PEQEPLSPNGR AcD3 100% free AVT1_YEAST Vacuolar amino acid transporter A6ZWL no PIDQE PIDQEKLAKLQKLSANNKVGGTR prop / NH2- / AcD3 100% free NACB1 _ YEAS7 Nascent polypeptide-associated complex subunit beta-1 - Saccharomyces cerevisiae (strain YJM789) (Baker's yeast Q02642 (2-24) Q no PITIK PITIKSR AcD3 100% free TSC13_YEAST Enoyl reductase TSC P no PKETP PKETPSKAAADALSDLEIKDSKSNLNKELETLR AcD3 100% free RIR2_YEAST Ribonucleoside-diphosphate reductase small chain P X no PKLVL PKLVLVR prop / NH2- / AcD3 100% free PMG1_YEAST Phosphoglycerate mutase P no PLNKS PLNKSNIR prop / AcD3 100% free RAS2_YEAST Ras-like protein 2 precursor P no PLPPS PLPPSTLNQKSNR prop / AcD3 100% free CSK22_YEAST Casein kinase II subunit alpha' P no PNASQ PNASQVYR AcD3 100% free THRC_YEAST Threonine synthase A6ZR no PQNDY PQNDYIER AcD3 100% free Ribosome biogenesis protein NSA2 - Saccharomyces cerevisiae NSA2_YEAS7 (strain YJM789) (Baker's yeast) P40078 (2-9) P ? PQSFT PQSFTSIAR prop free- QCR7_YEAST Cytochrome b-c1 complex subunit P no PSTLT PSTLTINGKAPIVAYAELIAAR AcD3 100% free SYEC_YEAST Glutamyl-tRNA synthetase, cytoplasmic P SAATA SAATATAAPVPPPVGISNLPNQR prop / Ace / AcD3 ynata/hnata (partial Ac) CDC12_YEAST Cell division control protein P SAGIG SAGIGESR AcD3 ynata/hnata (partial Ac) BIM1_YEAST Protein BIM A6ZT SAIPE SAIPENANVTVLNKNEKKAR prop / Ace ynata/hnata (partial Ac) NACA_YEAS7 Nascent polypeptide-associated complex subunit alpha P38879 (2-21) P0C0W SAKAQ SAKAQNPMR prop / Ace ynata/hnata (partial Ac) RL11A_YEAST 60S ribosomal protein L11-A P SAKQQ SAKQQLR prop / Ace / AcD3 ynata/hnata (partial Ac) ETFB_YEAST Probable electron transfer flavoprotein subunit beta P SALPE SALPEEVNR AcD3 ynata/hnata (partial Ac IMB3_YEAST Importin subunit beta P ? SANEF SANEFYSSGQQGQYNQQNNQER Ace ynata/hnata YNU8_YEAST Uncharacterized protein YNL208W Q SANLD SANLDKSLDEIIGSNKAGSNR Ace / AcD3 ynata/hnata (partial Ac) YRA1_YEAST RNA annealing protein YRA P (*) (*) SAPAA SAPAANGEVPTFKLVLVGDGGTGKTTFVKR AcD3 ynata/hnata (partial Ac) GSP1_YEAST GTP-binding nuclear protein GSP1/CNR P SAPAQ SAPAQNYKIADISLAAFGR prop / Ace / AcD3 ynata/hnata (partial Ac) SAHH_YEAST Adenosylhomocysteinase P ? SAPEA SAPEAQQQKR prop free- RS2_YEAST 40S ribosomal protein S P SATLF SATLFNNIELLPPDALFGIKQR AcD3 ynata/hnata (partial Ac) AATC_YEAST Aspartate aminotransferase, cytoplasmic P SATLK SATLKDYLNKR AcD3 ynata/hnata (partial Ac) LSM8_YEAST U6 snrna-associated Sm-like protein LSm Q96VH SAVAT SAVATYLKTLTAR Ace / AcD3 ynata/hnata (partial Ac) YC026_YEAST Uncharacterized protein YCL026C-B P SDAQF SDAQFDAALDLLR Ace / AcD3 ynata/hnata (partial Ac) CAPZB_YEAST F-actin-capping protein subunit beta P SDAVT SDAVTIR prop / Ace / AcD3 ynata/hnata (partial Ac) RS24_YEAST 40S ribosomal protein S

4 P ? SDEEH SDEEHTFENADAGASATYPMQCSALR Ace ynata/hnat IF5A1_YEAST Eukaryotic translation initiation factor 5A P SDEEH SDEEHTFETADAGSSATYPMQCSALR prop / Ace / AcD3 ynata/hnata (partial Ac IF5A2_YEAST Eukaryotic translation initiation factor 5A P SDFQK SDFQKEKVEEQEQQQQQIIKIR prop / Ace / AcD3 ynata/hnata (partial Ac) RS20_YEAST 40S ribosomal protein S P SDIKQ SDIKQLLKEAKQELTNR AcD3 ynata/hnata (partial Ac) SKI3_YEAST Superkiller protein Q ? SDILN SDILNVSQQR Ace ynata/hnata PNPH_YEAST Purine nucleoside phosphorylase Q SDINE SDINEIEIPSR AcD3 ynata/hnata (partial Ac) YO052_YEAST AN1-type zinc finger protein YOR052C P (*) SDPVE SDPVELLKR prop / Ace ynata/hnata (partial Ac) SEC17_YEAST Alpha-soluble NSF attachment protein P ? SDSNQ SDSNQGNNQQNYQQYSQNGNQQQGNNR Ace- Ac- ERF2_YEAST Eukaryotic peptide chain release factor GTP-binding subunit P SDSQQ SDSQQSIKVLEELFQKLSVATADNR Ace / AcD3 ynata/hnata (partial Ac) EF3A_YEAST Elongation factor 3A P SDVLS SDVLSQYKGCSVR AcD3 ynata/hnata (partial Ac) YGU6_YEAST Uncharacterized protein YGL196W Q SDVTQ SDVTQQKKR AcD3 ynata/hnata (partial Ac) RRP9_YEAST Ribosomal RNA-processing protein Q9P ? SEDLS SEDLSPTSSR Ace ynata/hnata IGO2_YEAST Protein IGO P SEECI SEECIENPER AcD3 ynata/hnata (partial Ac) THI80_YEAST Thiamine pyrophosphokinase A6ZTA3 2 13? SEEFI SEEFIAVSTLAR Ace ynata/hnata RIX1_YEAS7 Pre-rRNA-processing protein RIX1 P38883 (2-13) P SEEGP SEEGPQVKIR Ace / AcD3 ynata/hnata (partial Ac) RPB3_YEAST DNA-directed RNA polymerase II subunit RPB P SEEKT SEEKTYKR AcD3 ynata/hnata (partial Ac) PMT1_YEAST Dolichyl-phosphate-mannose--protein mannosyltransferase P ? SEEPP SEEPPSDQVNSLR Ace ynata/hnata SFT2_YEAST Protein transport protein SFT P ? SEFLN SEFLNENPDILEENQLPTR Ace- Ac- YHS2_YEAST UPF0195 protein YHR122W P SEGFS SEGFSSSSIQELYQSLKEITNNADVELFEDR AcD3 ynata/hnata (partial Ac) SKI2_YEAST Antiviral helicase SKI P X (*) (*) SEITL SEITLGKYLFER Ace / AcD3 ynata/hnata (partial Ac) PDC1_YEAST Pyruvate decarboxylase isozyme 1 P16467 (2-13)^AP26263 (2-13) P SEKFP SEKFPPLEDQNIDFTPNDKKDDDTDFLKR AcD3 ynata/hnata (partial Ac) CLC1_YEAST Clathrin light chain P SEKPQ SEKPQQEEQEKPQSR AcD3 ynata/hnata (partial Ac) LAH1_YEAST La protein homolog P ? SELFG SELFGVLKSNAGR Ace- Ac- MPP10_YEAST U3 small nucleolar RNA-associated protein MPP P SELTE SELTEAEKR prop / Ace / AcD3 ynata/hnata (partial Ac) GET2_YEAST Golgi to ER traffic protein SENNEFQSVTESTTAPTTSNPYGPNPADYLSN Q SENNE VKNFQLIDSTLR prop / Ace / AcD3 ynata/hnata (partial Ac) HOSM_YEAST Homocitrate synthase, mitochondrial precursor Q ? SENNG SENNGNPLDLSSLR Ace- Ac- MAK21_YEAST Ribosome biogenesis protein MAK P ? SENTT SENTTAPSDNITNEQR Ace ynata/hnata SUM1_YEAST Suppressor of mar1-1 protein P ? SEPEF SEPEFQQAYEEVVSSLEDSTLFEQHPEYR Ace ynata/hnata DHE4_YEAST NADP-specific glutamate dehydrogenase Q SEQES SEQESDEVKR prop / Ace / AcD3 ynata/hnata (partial Ac) BUG1_YEAST Binder of USO1 and GRH1 protein P ? SEQLR SEQLR prop free- TRP_YEAST Tryptophan synthase P ? SESPM SESPMFAANGMPKVNQGAEEDVR Ace ynata/hnata AROG_YEAST Phospho-2-dehydro-3-deoxyheptonate aldolase, tyrosine-inhibited Q SEVAP SEVAPEEIIENADGSR prop / Ace / AcD3 ynata/hnata (partial Ac) EIF3G_YEAST Eukaryotic translation initiation factor 3 RNA-binding subunit P SEVIE SEVIEGNVKIDR prop / Ace / AcD3 ynata/hnata (partial Ac) FKBP_YEAST FK506-binding protein P ? SFDDL SFDDLHKATER Ace ynata/hnata HXKG_YEAST Glucokinase GLK P ? SFFGL SFFGLENSGNAR Ace ynata/hnata PAT1_YEAST DNA topoisomerase 2-associated protein PAT SFIKNLLFGGVKTSEDPTGLTGNGASNTNDSN Q SFIKN KGSEPVVAGNFFPR AcD3 ynata/hnata (partial Ac) DAP1_YEAST Damage response protein P SFLSK SFLSKLSQIR AcD3 ynata/hnata (partial Ac) SPT2_YEAST Protein SPT P SFNSP SFNSPFFDFFDNINNEVDAFNR Ace / AcD3 ynata/hnata (partial Ac) HSP26_YEAST Heat shock protein P SGAAA SGAAAASAAGYDR prop / Ace / AcD3 ynata/hnata (partial Ac) PSA6_YEAST Proteasome component C7-alpha P no SGELA SGELANYKR AcD3 100% free CDC28_YEAST Cell division control protein P SGETF SGETFEFNIR Ace / AcD3 ynata/hnata (partial Ac) UBP6_YEAST Ubiquitin carboxyl-terminal hydrolase P ? SGGKG SGGKGGKAGSAAKASQSR Ace- Ac- H2A1_YEAST Histone H2A.1 P04912 (2-19) P ? SGIID SGIIDASSALR Ace ynata/hnata CDC11_YEAST Cell division control protein P SGNGA SGNGAQGTKFR prop / Ace / AcD3 ynata/hnata (partial Ac) RL23_YEAST 60S ribosomal protein L P ? SGNQM SGNQMAMGSEQQQTVGSR Ace ynata/hnata YFH3_YEAST Uncharacterized protein YFR003C P ? SGSTE SGSTESKKQPR Ace ynata/hnata DPH1_YEAST Diphthamide biosynthesis protein P SIAEF SIAEFAYKEKPETLVLFDVDGTLTPAR AcD3 ynata/hnata (partial Ac) PMM_YEAST Phosphomannomutase P SITKT SITKTELDGILPLVAR prop / Ace / AcD3 ynata/hnata (partial Ac) PUR7_YEAST Phosphoribosylaminoimidazole-succinocarboxamide synthase P SKATY SKATYKER prop / Ace / AcD3 ynata/hnata (partial Ac) PYRF_YEAST Orotidine 5'-phosphate decarboxylase P SKITS SKITSSQVR prop / Ace / AcD3 ynata/hnata (partial Ac) RL1_YEAST 60S ribosomal protein L P SKKFS SKKFSSKNSQR prop / Ace ynata/hnata (partial Ac) LTV1_YEAST Protein LTV P ? SKKLS SKKLSLEER Ace ynata/hnata YJ9C_YEAST Uncharacterized protein YJR134C P SKVAQ SKVAQQLKFLNKISATTR AcD3 ynata/hnata (partial Ac) RM49_YEAST Mitochondrial 54S ribosomal protein MRP P ? SKVIK SKVIKPPGQAR prop free- RPA2_YEAST DNA-directed RNA polymerase I subunit RPA P ? SKVPR SKVPR Ace ynata/hnata MMS2_YEAST Ubiquitin-conjugating enzyme variant MMS P SLDFN SLDFNTLAQNFTQFYYNQFDTDR Ace / AcD3 ynata/hnata (partial Ac) NTF2_YEAST Nuclear transport factor Q ? SLISA SLISAVEDR Ace- Ac- YMS1_YEAST Uncharacterized protein YMR031C P SLISI SLISILSPLITSEGLDSR AcD3 ynata/hnata (partial Ac) GUP1_YEAST Glycerol uptake protein P SLLID SLLIDSVPTVAYKDQKPGTSGLR prop / Ace ynata/hnata (partial Ac) PGM1_YEAST Phosphoglucomutase Q SLPGS SLPGSKLEEAR AcD3 ynata/hnata (partial Ac) RPN6_YEAST 26S proteasome regulatory subunit RPN P ? SLQLL SLQLLNPKAESLR prop free- TCPZ_YEAST T-complex protein 1 subunit zeta P SLSSK SLSSKLSVQDLDLKDKR prop / Ace / AcD3 ynata/hnata (partial Ac) PGK_YEAST Phosphoglycerate kinase

5 O SMSST SMSSTVIKFR AcD3 ynata/hnata (partial Ac) RPN13_YEAST 26S proteasome regulatory subunit RPN Q ? SNDGS SNDGSKILNYTPVSKIDEIVEISR AcD3 free- YM00_YEAST Putative aldehyde dehydrogenase-like protein YMR110C P ? SNEIE SNEIELLQKQVSELQDLVKKQSLIISKTGER Ace ynata/hnata MRP8_YEAST Uncharacterized protein MRP P ? SNIVG SNIVGIEYNR Ace ynata/hnata RPAC1_YEAST DNA-directed RNA polymerases I and III subunit RPAC P SNKLF SNKLFR Ace / AcD3 ynata/hnata (partial Ac) MANA_YEAST Mannose-6-phosphate isomerase Q SNLYK SNLYKIGTETR AcD3 ynata/hnata (partial Ac) YD063_YEAST Uncharacterized protein YDR063W P SNTLF SNTLFDDIFQVSEVDPGR Ace / AcD3 ynata/hnata (partial Ac) RPAB3_YEAST DNA-directed RNA polymerases I, II, and III subunit RPABC Q ? SNTSE SNTSEDNITVR Ace ynata/hnata HPA2_YEAST Histone acetyltransferase HPA P ? SNVVQ SNVVQAR prop free- RS14A_YEAST 40S ribosomal protein S14-A Q SPLNV SPLNVGIVGTGIFAR AcD3 ynata/hnata (partial Ac) YM94_YEAST Uncharacterized protein YMR315W Q no SPMKV SPMKVAVVGASGKVGR prop / AcD3 100% free YMY0_YEAST Uncharacterized protein YMR090W P no SPPVY SPPVYSDISR prop / AcD3 100% free VDAC1_YEAST Outer mitochondrial membrane protein porin Q SQAVN SQAVNAKKR prop / Ace / AcD3 ynata/hnata (partial Ac) RS15_YEAST 40S ribosomal protein S P SQGTL SQGTLYANFR prop / Ace / AcD3 ynata/hnata (partial Ac) EF1G1_YEAST Elongation factor 1-gamma P SQGTL SQGTLYINR prop / Ace ynata/hnata (partial Ac) EF1G2_YEAST Elongation factor 1-gamma P ? SQIAQ SQIAQEMTVSLR Ace ynata/hnata PFD1_YEAST Prefoldin subunit P ? SQLFN SQLFNNSR Ace ynata/hnata TCPA_YEAST T-complex protein 1 subunit alpha Q SQLPT SQLPTDFASLIKR prop / Ace / AcD3 ynata/hnata (partial Ac) DCPS_YEAST Scavenger mrna-decapping enzyme DcpS P ? SQNIQ SQNIQIGTR Ace ynata/hnata KNS1_YEAST Dual specificity protein kinase KNS Q9URQ SQNNT SQNNTISSMNPER prop / Ace ynata/hnata (partial Ac) HTL1_YEAST High temperature lethal protein P SQNVY SQNVYIVSTAR prop / Ace / AcD3 ynata/hnata (partial Ac) THIL_YEAST Acetyl-CoA acetyltransferase P SQQVG SQQVGNSIR prop / Ace / AcD3 ynata/hnata (partial Ac) RHO1_YEAST GTP-binding protein RHO1 precursor P SQVNT SQVNTSQGPVAQGR prop / Ace ynata/hnata (partial Ac) TREA_YEAST Neutral trehalase P ? SQVQS SQVQSPLTATNSGLAVNNNTMNSQMPNR Ace- Ac- KC12_YEAST Casein kinase I homolog P SQVYF SQVYFDVEADGQPIGR prop / Ace / AcD3 ynata/hnata (partial Ac) CYPH_YEAST Peptidyl-prolyl cis-trans isomerase P ? SSAAE SSAAEKKPASKAPAEKKPAAKKTSTSVDGKKR prop free- H2B2_YEAST Histone H2B P SSAIT SSAITALTPNQVNDELNKMQAFIR Ace / AcD3 ynata/hnata (partial Ac) VATE_YEAST Vacuolar proton pump subunit E P ? SSAPL SSAPLLQR Ace ynata/hnata VTC1_YEAST Vacuolar transporter chaperone P SSGLV SSGLVLENTAR Ace / AcD3 ynata/hnata (partial Ac) SYLC_YEAST Leucyl-tRNA synthetase, cytoplasmic P ? SSISL SSISLKEIIPPQPSTQR prop / AcD3 free- AIP1_YEAST Actin-interacting protein Q ? SSIYK SSIYKALAGKSKDNKSEKKQGNVKQFMNKQR Ace ynata/hnata BRX1_YEAST Ribosome biogenesis protein BRX P SSLCL SSLCLQR prop / Ace / AcD3 ynata/hnata (partial Ac) UBC9_YEAST SUMO-conjugating enzyme UBC Q SSLLI SSLLISYESDFKTTLEQAKASLAEAPSQPLSQR AcD3 ynata/hnata (partial Ac) VTI1_YEAST P SSNLT SSNLTEEQIAEFKEAFALFDKDNNGSISSSELA TVMR t-snare VTI1 (Qb-SNARE VTI1) (Vesicle transport v-snare protein VTI1)(VPS10-interacting protein 1) AcD3 ynata/hnata (partial Ac) CALM_YEAST Calmodulin P (*)? SSNNS SSNNSGLSAAGEIDESLYSR Ace ynata/hnata UBA1_YEAST Ubiquitin-activating enzyme E P ? SSNTS SSNTSSVMSSPR Ace ynata/hnata NUP1_YEAST Nucleoporin NUP P SSPTP SSPTPPGGQR prop / Ace ynata/hnata (partial Ac) SC6B1_YEAST Protein transport protein SBH P ? SSQPS SSQPSFVTIR Ace ynata/hnata GOS1_YEAST Protein transport protein GOS P SSSES SSSESIR prop / AcD3 ynata/hnata (partial Ac) KAD1_YEAST Adenylate kinase cytosolic P SSSIF SSSIFGPLTGFLER AcD3 ynata/hnata (partial Ac) YN8B_YEAST Uncharacterized protein YNR021W P SSSKF SSSKFEEVINKIINDSPPGELR Ace / AcD3 ynata/hnata (partial Ac) CAPZA_YEAST F-actin-capping protein subunit alpha P (*) (*) SSSKK SSSKKLAGLR Ace / AcD3 ynata/hnata (partial Ac) PYC2_YEAST Pyruvate carboxylase P SSSQQ SSSQQIAKNAR prop / Ace / AcD3 ynata/hnata (partial Ac) PROA_YEAST Gamma-glutamyl phosphate reductase Q STAEF STAEFAQLLENSILSPDQNIR prop / AcD3 ynata/hnata (partial Ac IMB1_YEAST Importin subunit beta P ? STDTM STDTMYFNSSR Ace ynata/hnata EDC1_YEAST Enhancer of mrna-decapping protein P STEEQ STEEQNGVPLQR prop / Ace ynata/hnata (partial Ac) MCK1_YEAST Protein kinase MCK Q STEKI STEKILTPESQLKKTKAQQKTAEQIAAER Ace / AcD3 ynata/hnata (partial Ac) RL7B_YEAST 60S ribosomal protein L7-B (L6) (YL8) (RP11) P STELT STELTVQSER prop / Ace / AcD3 ynata/hnata (partial Ac) RS11_YEAST 40S ribosomal protein S P STFGK STFGKLFR prop / Ace / AcD3 ynata/hnata (partial Ac) AROC_YEAST Chorismate synthase P STFSA STFSASDFNSER Ace / AcD3 ynata/hnata (partial Ac) YE05_YEAST Uncharacterized protein YER175C Q ? STIST STISTTTAPEFIR Ace ynata/hnata IWR1_YEAST Protein IWR Q3E STKAQ STKAQNPMR prop / Ace / AcD3 ynata/hnata (partial Ac) RL11B_YEAST 60S ribosomal protein L11-B P ? STLLK STLLKSAKSIVPLMDR Ace ynata/hnata CH10_YEAST 10 kda heat shock protein, mitochondrial Q ? STMST STMSTPAAEQR Ace ynata/hnata LUC7_YEAST Protein LUC P STPFG STPFGLDLGNNNSVLAVAR prop / Ace / AcD3 ynata/hnata (partial Ac) HSP7F_YEAST Heat shock protein homolog SSE1 P32590 (2-20) P STSVP STSVPVKKALSALLR Ace / AcD3 ynata/hnata (partial Ac) AGE2_YEAST Protein AGE P STTAS STTASTPSSLR prop / Ace / AcD3 ynata/hnata (partial Ac) OTC_YEAST Ornithine carbamoyltransferase P SVEDI SVEDIKKAR Ace / AcD3 ynata/hnata (partial Ac) SYG_YEAST Glycyl-tRNA synthetase Q ? SVEEK SVEEKKR Ace ynata/hnata NHP10_YEAST Non-histone protein P (*)? SVKPI SVKPIDNYITNSVR Ace ynata/hnata SRP21_YEAST Signal recognition particle subunit SRP P ? SVQIF SVQIFGDQVTEER Ace ynata/hnata TCPB_YEAST T-complex protein 1 subunit beta P SVSLE SVSLEQTLGFR AcD3 ynata/hnata (partial Ac) LSM12_YEAST Protein LSM P SWEGF SWEGFKKAINR AcD3 ynata/hnata (partial Ac) RV161_YEAST Reduced viability upon starvation protein A6ZTF ? SYGTI SYGTINDMNESVTNYR Ace ynata/hnata ATG22_YEAS7 Autophagy-related protein 22 P25568 (2-17) P ? SYSAA SYSAADNLQDSFQR Ace ynata/hnata SER33_YEAST D-3-phosphoglycerate dehydrogenase

6 O ? SYTDN SYTDNPPQTKR Ace ynata/hnata CET1_YEAST mrna-capping enzyme subunit beta P no TAAKP TAAKPNPYAAKPGDYLSNVNNFQLIDSTLR AcD3 100% free HOSC_YEAST Homocitrate synthase, cytosolic isozyme P no TAGSA TAGSAPPVDYTSLKKNFQPFLSR prop / AcD3 100% free ARO9_YEAST Aromatic amino acid aminotransferase P no TAIED TAIEDILQITTDPSDTR AcD3 100% free UFD2_YEAST Ubiquitin conjugation factor E P no TAQQA TAQQAPKWYPSEDVAAPKKTR AcD3 100% free RL6B_YEAST 60S ribosomal protein L6-B P no TASSN TASSNDDDLIFECYSDPELKR AcD3 100% free YG2W_YEAST Uncharacterized protein YGR111W Q TASTP TASTPSNVMTLFLLR AcD3 ynata/hnata (partial Ac) PMG2_YEAST Phosphoglycerate mutase P TDYIL TDYILPGPKALSQFR prop / Ace / AcD3 ynata/hnata (partial Ac) PUR4_YEAST Phosphoribosylformylglycinamidine synthase P (*) (*) TEFEL TEFELPPKYITAANDLR AcD3 ynata/hnata (partial Ac) GLY1_YEAST Low specificity L-threonine aldolase P TEFKA TEFKAGSAKKGATLFKTR prop / Ace / AcD3 <5% Ac (ynata/hnata)* CYC1_YEAST Cytochrome c iso P ? TGMES TGMESGENLENMEDILAR Ace ynata/hnata OTU2_YEAST OTU domain-containing protein P no TISNL TISNLLKQR prop / AcD3 100% free ACH1_YEAST Acetyl-CoA hydrolase A6ZUA no TKEEI TKEEIADKKR AcD3 100% free ATP-dependent RNA helicase DBP3 - Saccharomyces cerevisiae DBP3 _ YEAS7 (strain YJM789) (Baker's yeast) P20447 (2-11) P no TKKSF TKKSFVSSPIVR AcD3 100% free ATC6_YEAST Probable cation-transporting ATPase P no TKSEQ TKSEQQADSR prop / AcD3 100% free CBS_YEAST Cystathionine beta-synthase P (*) (*) TLPES TLPESKDFSYLFSDETNAR AcD3 ynata/hnata (partial Ac) ARO8_YEAST Aromatic amino acid aminotransferase P no TLSKY TLSKYSKPTLNDPNLFR AcD3 100% free UGA2_YEAST Succinate-semialdehyde dehydrogenase [NADP+] Q no TLVTP TLVTPLQFSTVQPNLYR AcD3 100% free OCA6_YEAST Putative tyrosine-protein phosphatase OCA P TQFTD TQFTDIDKLAVSTIR prop / AcD3 ynata/hnata (partial Ac) TKT1_YEAST Transketolase P no TQLKY TQLKYLLLVSR AcD3 100% free AP1S1_YEAST AP-1 complex subunit sigma P ? TSIDI TSIDINNLQNTFQQAMNMSGSPGAVCTSPTQS FMNTVPQR Ace- Ac- SERA_YEAST D-3-phosphoglycerate dehydrogenase P TSIGT TSIGTGYDLSNSVFSPDGR prop / AcD3 ynata/hnata (partial Ac) PSA3_YEAST Proteasome component C Q TSKPS TSKPSTSDGR prop / Ace / AcD3 ynata/hnata (partial Ac) XPP_YEAST Putative Xaa-Pro aminopeptidase P TTTAQ TTTAQDNSPKKR prop / Ace / AcD3 ynata/hnata (partial Ac) TPS2_YEAST Trehalose-phosphatase Q ? TTTST TTTSTTSVDGR prop free- PBP4_YEAST Protein PBP P no TVFLG TVFLGIYR AcD3 100% free SLA1_YEAST Cytoskeleton assembly control protein SLA P no TVKTG TVKTGIAIGLNKGKKVTSMTPAPKISYKKGAAS NR AcD3 100% free RL36A_YEAST 60S ribosomal protein L36-A P ? TVPYL TVPYLNSNR prop / AcD3 free- CISY2_YEAST Citrate synthase, peroxisomal Q3E no VAFLE VAFLELTSDVSQPFVIPSLSPVSQPSSR AcD3 100% free YP036_YEAST Uncharacterized protein YPR036W-A P X no VAFTV VAFTVDQMR AcD3 100% free EF2_YEAST Elongation factor P no VALIS VALISKKR prop / AcD3 100% free RS3_YEAST 40S ribosomal protein S P no VELTE VELTEIKDDVVQLDEPQFSR AcD3 100% free TOM22_YEAST Mitochondrial import receptor subunit TOM P ? VFDLK VFDLKR prop free- HIS8_YEAST Histidinol-phosphate aminotransferase Q no VGGAL VGGALICKYLPR AcD3 100% free TRM3_YEAST trna guanosine-2'-o-methyltransferase TRM Q no VIKED VIKEDCINNLAR AcD3 100% free PIB1_YEAST E3 ubiquitin-protein ligase PIB P no VKETK VKETKLYDLLGVSPSANEQELKKGYR prop / AcD3 100% free SIS1_YEAST Protein SIS P no VKLAE VKLAEFSR prop / AcD3 100% free SEC31_YEAST Protein transport protein SEC P no VLADL VLADLGKR prop / AcD3 100% free SRP54_YEAST Signal recognition particle subunit SRP Q no VLAME VLAMESR AcD3 100% free MCE1_YEAST mrna-capping enzyme subunit alpha Q no VLLIS VLLISEIKDIAKR AcD3 100% free NOC4_YEAST Nucleolar complex protein P no VLSDK VLSDKELFAINKKAVEQGFNVKPR AcD3 100% free VATB_YEAST Vacuolar ATP synthase subunit B Q no VLVKQ VLVKQVR AcD3 100% free YP088_YEAST Putative aryl-alcohol dehydrogenase YPL088W P no VNVPK VNVPKTR AcD3 100% free RL44_YEAST 60S ribosomal protein L Q no VPAES VPAESNAVQAKLAKTLQR AcD3 100% free YO164_YEAST UPF0363 protein YOR164C Q no VQAIK VQAIKLNDLKNR AcD3 100% free BCP1_YEAST Protein BCP P no VQAVA VQAVAVLKGDAGVSGVVKFEQASESEPTTVS YEIAGNSPNAER AcD3 100% free SODC_YEAST Superoxide dismutase [Cu-Zn] P no VQSAV VQSAVLGFPR prop / AcD3 100% free METE _YEAST 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase P no VSTPE VSTPELKKYMDKKILLNINGSR AcD3 100% free RUXG_YEAST Small nuclear ribonucleoprotein G P no VTSNV VTSNVVLVSGEGER prop / AcD3 100% free SKP1_YEAST Suppressor of kinetochore protein P no VTYAG VTYAGKLVLAPMVR AcD3 100% free DUS2_YEAST trna-dihydrouridine synthase Q ? VVKAI VVKAIAR prop free- RM51_YEAST Mitochondrial 54S ribosomal protein L P no VYTPS VYTPSKGPR prop / AcD3 100% free LEU2 _YEAST 3-isopropylmalate dehydratase

7 Table S1 B. Compilation of of the N-terminal acetylation status of 616 yeast N-termini based on this study and previous studies (a) Acetylation: +, acetylated; -, not acetylated; ± partially acetylated; +(-N.D.), acetylated (non acetylated form experimentally not determined); -(+N.D.), non acetylated (acetylated form experimentally not determined); ± & -, reported to be acetylated and not acetylated; % acetylation of some proteins are presented. (b) Nat column: A, NatA substrate; B, NatB substrate; C, NatC substrate; D, NatD (Nat4p) substrate -, not acetylated; N.D., not determined. The assignments of human NatA, NatB and NatC substrates are based primarily on analogy to yeast proteins. Proteins with Ac-MQ- termini have been tentatively assigned as NatB substates. (c) Methods: CO, COFRADIC, N-terminal peptides were isolated prior to LC-MS/MS analysis by COFRADIC; MS, acetylation was determined by MS; 2D, acetylation was determined by detection of a shift of a corresponding protein spot on a 2D-gel in a mutant strain as compared to a control strain; HPLC, acetylation was determined by HPLC separation (i.e. comparative chromatographic analysis) in conjunction with peptide sequencing; a, N-terminal Edman sequencing. (14) referring to this study. (15) referring to Polevoda B, Hoskins J, Sherman F, unpublished. (*) N-terminus identified with and without initiator Met attached. Processed N-termini (such as the N- termini from mitochondrial proteins) are omitted from this list. References are indicated at the bottom of the table. P1'-P5' Acetylation (a) Nat (b) Gene accession UNIPROT Description Ref Methods (c) AAEKI ± & - A RPL7A P05737 RL7A_YEAST 60S ribosomal protein L7-A 14,1,5 2D, MS, CO AAGEQ + A GUA1 P38625 GUAA_YEAST GMP synthase [glutamine-hydrolyzing] 6,8 2D AAGVP - - RKI1 Q12189 RPIA_YEAST Ribose-5-phosphate isomerase 14 CO AAQKS - - RPL39 P04650 RL39_YEAST 60S ribosomal protein L39 14,1 MS, CO AARPQ + A CCT5 P40413 TCPE_YEAST T-complex protein 1 subunit epsilon 6,8 2D AASVP 51 % A SEB2 P52871 SC6B2_YEAST Protein transport protein SEB2 14 CO AATKE ± A NHP6B P11633 NHP6B_YEAST Non-histone chromosomal protein 6B 14 CO ADITD ± A PAB1 P04147 PABP_YEAST Polyadenylate-binding protein, cytoplasmic and nuclear 6,10,8 2D ADLQK +(-N.D.) A NUP53 Q03790 NUP53_YEAST Nucleoporin NUP53 14 CO ADQEN - - COX12 Q COX1 2_YEAST Cytochrome c oxidase polypeptide VIb 9 a ADYSS 19 % A THO1 P40040 THO1_YEAST Protein THO1 14 CO AEASI 85 % A GLN1 P32288 GLNA_YEAST Glutamine synthetase 14,3,6,10,8 2D, CO AEEST +(-N.D.) A RDI1 Q12434 GDIR_YEAST Rho GDP-dissociation inhibitor 14 CO AEGVF + A SSB1 P11484 HSP75_YEAST Heat shock protein SSB1 3,6,10,8 2D AEGVF + A SSB2 P40150 HSP76_YEAST Heat shock protein SSB2 3,6,10,8 2D AEKEE + A VPH1 P32563 VPH1_YEAST Vacuolar ATP synthase subunit a, vacuolar isoform 14,1 2D, CO AESHR + A RPL33A P05744 RL33A_YEAST 60S ribosomal protein L26-A 1 MS AFLNI 10 % A APD1 P38281 APD1_YEAST Actin patches distal protein 1 14 CO AFQKD <5% A RPL5 P26321 RL5_YEAST 60S ribosomal protein L5 14 CO AGAIE 43 % A VMA1 P17255 VATA_YEAST Vacuolar ATP synthase catalytic subunit A 14,3,6,8 2D, CO AGENP 9 % A ESF1 Q06344 ESF1_YEAST Pre-rRNA-processing protein ESF1 14 CO AGETF ± A HSC82 P15108 HSC82_YEAST ATP-dependent molecular chaperone HSC82 3,6, 10, 8 2D AGKKI 87% (± & -) A HOM2 P13663 DHAS_YEAST Aspartate-semialdehyde dehydrogenase 14,6,1 0,8 2D, CO AGLKD - - RPL31A P0C2H8 (*) RL31A_YEAST 60S ribosomal protein L31-A 14,1 MS, CO AGLKD - - RPL31B P0C2H9 (*) RL31B_YEAST 60S ribosomal protein L31-B 14,1 MS, CO AGNAN 33 % A YVH1 Q02256 PVH1_YEAST Tyrosine-protein phosphatase YVH1 14 CO AGQVL - - ADE3 P07245 C1TC_YEAST C-1-tetrahydrofolate synthase, cytoplasmic 14,9 a, CO AGSQL ± A NOP14 Q99207 NOP14_YEAST Nucleolar complex protein CO AGTFL ± A SAM1 P10659 METK1_YEAST S-adenosylmethionine synthetase 1 3, 6,9, 8 2D, a AGVKA - - RPL35B P39741 RL35_YEAST 60S ribosomal protein L35 14 CO AGVSV - - RPS19B P07281 RS19B_YEAST 40S ribosomal protein S19-B 14,1 MS, CO AGWDI - - SAR1 P20606 SAR1_YEAST Small COPII coat GTPase SAR1 6,10,8 2D AHENV - - RPS29A P41057 (*) RS29A_YEAST 40S ribosomal protein S29-A 1 MS AHENV - - RPS29B P41058 (*) RS29B_YEAST 40S ribosomal protein S29-B 1 MS AHIPE - - LCB1 P25045 LCB1_YEAST Serine palmitoyltransferase D AIDYS - - CDC37 P06101 CDC37_YEAST Hsp90 co-chaperone Cdc37 6,8 2D AIKSL - - MKT1 P40850 MKT1_YEAST Protein MKT1 14 CO AKEST - - CYC7 P00045 CYC7_YEAST Cytochrome c iso-2 14,9 a, CO AKFLK - - RPL27A P0C2H6 (*) RL27A_YEAST 60S ribosomal protein L27-A 14,1 MS, CO AKFLK - - RPL27B P0C2H7 (*) RL27B_YEAST 60S ribosomal protein L27-B 14,1 MS, CO AKKKS - - UTP14 Q04500 UTP14_YEAST U3 small nucleolar RNA-associated protein CO AKKVA - - GLG2 P47011 GLG2_YEAST Glycogen synthesis initiator protein GLG2 14 CO AKQSL - - RPL26A P05743 (*) RL26A_YEAST 60S ribosomal protein L26-A 14 CO AKQSL - - RPL26B P53221 (*) RL26B_YEAST 60S ribosomal protein L26-B 14 CO AKSKN - - RPL29 P05747 RL29_YEAST 60S ribosomal protein L29 1 MS AKTIK - - YGR026W P53217 YG1I_YEAST Uncharacterized membrane protein YGR026W 14 CO AKVHG - - RPS30A Q12087 RS30_YEAST 40S ribosomal protein S30 1 MS ALGNE - - RPF1 P38805 RPF1_YEAST Ribosome production factor 1 14 CO AMNTG +(-N.D.) A MON2 P48563 MON2_YEAST Protein MON2 14 CO ANDLV +(-N.D.) A RPS14B P39516 RS14B_YEAST 40S ribosomal protein S14-B 14 CO ANLRT - - RPL19A P05735 RL19_YEAST 60S ribosomal protein L19 1,5 2D, MS ANLRT - - RPL19B P05735 RL19_YEAST 60S ribosomal protein L19 5 2D, a ANYTK - - ADE1 7 P38009 PUR92_YEAST Bifunctional purine biosynthesis protein ADE17 14 CO APGKK - - RPL8B P29453 RL8B_YEAST 60S ribosomal protein L8-B 5 2D, a APGKK (*) - - RPL8A P17076 RL8A_YEAST 60S ribosomal protein L8-A 14,1,5 2D, MS, CO APLTK - - PUF6 Q04373 PUF6_YEAST Protein PUF6 14 CO APNTS - - RPL22A P05749 RL22A_YEAST 60S ribosomal protein L22-A 1 MS APPKK - - TIF3 P34167 IF4B_YEAST Eukaryotic translation initiation factor 4B 6,8 2D APSAK - - RPL25 P04456 RL25_YEAST 60S ribosomal protein L25 14,1,5 2D, MS, CO APVKS - - RPL30 P14120 RL30_YEAST 60S ribosomal protein L30 1 MS APVTI - - YDR380W Q06408 ARO10_YEAST Transaminated amino acid decarboxylase 6,8 2D AQEEI + A SPE3 Q12074 SPEE_YEAST Spermidine synthase 6,10,8 2D AQFSD -(+N.D.) - TKL2 P33315 TKT2_YEAST Transketolase 2 14 CO ARDLQ - - GSY1 P23337 GYS1_YEAST Glycogen [starch] synthase isoform 1 9 a AREIT - - RPL38 P49167 RL38_YEAST 60S ribosomal protein L38 1 MS ARGPK - - RPS4A P05753 RS4_YEAST 40S ribosomal protein S4 1 MS ARRPA - - RPL10 P41805 RL10_YEAST 60S ribosomal protein L10 1 MS ARTFF - - TPI1 P00942 TPIS_YEAST Triosephosphate isomerase 3,6,10,8 2D ARYGA - - RPL17A P05740 RL17A_YEAST 60S ribosomal protein L17-A 1 MS ASEKE 35 % A FPP1 P08524 FPPS_YEAST Farnesyl pyrophosphate synthetase 14 CO ASETE ± A HSP82 P02829 HSP82_YEAST ATP-dependent molecular chaperone HSP82 6,8 2D ASIGS 46 % A GCN20 P43535 GCN20_YEAST Protein GCN20 14,6,8 2D, CO ASLPH - - RPL32 P38061 RL32_YEAST 60S ribosomal protein L32 1 MS ASNEV 32 % A ASC1 P38011 GBLP_YEAST Guanine nucleotide-binding protein subunit beta-like protein 14 CO ASTAN + A YDR341C Q05506 SYRC_YEAST Arginyl-tRNA synthetase, cytoplasmic 6,10,8 2D ASTDF + A EFB1 P32471 EF1B_YEAST Elongation factor 1-beta 6,10,8 2D ASTSN + A YBR214W P38314 YB64_YEAST Uncharacterized protein YBR214W 14 CO ASVLL -(+N.D.) - DIB1 Q06819 DIB1_YEAST Spliceosomal protein DIB1 14 CO ATFNP +(-N.D.) A YDL173W Q12515 YD173_YEAST Uncharacterized protein YDL173W 14 CO

8 ATIAP 52 % A URA2 P07259 PYR1_YEAST Protein URA1 14 CO ATKQL +(-N.D.) A FAU1 P40099 FTHC_YEAST 5-formyltetrahydrofolate cyclo-ligase 14 CO ATLEE ± A RPT5 P33297 PRS6A_YEAST 26S protease regulatory subunit 6A 14 CO ATMET + A NBP2 Q12163 NBP2_YEAST NAP1-binding protein 2 14 CO ATPSN -(+N.D.) - HAS1 Q03532 HAS1_YEAST ATP-dependent RNA helicase HAS1 14 CO ATPST +(-N.D.) A TEM1 P38987 TEM1_YEAST Protein TEM1 14 CO AVGKN - - RPS1A P33442 (*) RS3A_YEAST 40S ribosomal protein S1-A 14,1,5 2D, MS, CO AVGKN - - RPS1B P23248 (*) RS3B_YEAST 40S ribosomal protein S1-B 14,5 2D, α, CO AVIKK -(+N.D.) - MYO3 P36006 MYO3_YEAST Myosin-3 14 CO AVKGL - - RIB4 P50861 RIB4_YEAST 6,7-dimethyl-8-ribityllumazine synthase 14 CO AVKTG ± & - A RPL36B O14455 RL36B_YEAST 60S ribosomal protein L36-B 14,1 MS, CO AVSKV - - ENO1 P00924 ENO1_YEAST Enolase 1 14,3,6,10,8 2D, CO AVSNN -(+N.D.) - YLR361C-A Q3E795 YL361_YEAST Uncharacterized protein YLR361C-A 14 CO AYADS - - VPS35 P34110 VPS35_YEAST Vacuolar protein sorting-associated protein CO CGIFA - - ASN1 P49089 ASNS1_YEAST Asparagine synthetase [glutamine-hydrolyzing] 1 14,6,8 2D, CO CGIFA - - ASN2 P49090 ASNS2_YEAST Asparagine synthetase 6,8 2D CGILG ± N.D. ADE4 P04046 PUR1_YEAST Amidophosphoribosyltransferase 6,8 2D DPELQ N.D. N.D. YMR074C Q04773 YMW4_YEAST Uncharacterized protein YMR074C 14 CO EFVAE(*) N.D. N.D. TPK2 P06245 KAPB_YEAST camp-dependent protein kinase type 2 14 CO GAYKY (*) - - RPL15A P05748 RL15A_YEAST 60S ribosomal protein L15-A 14,1,5 2D, MS, CO GEEHK - - CDC48 P25694 CDC48_YEAST Cell division control protein 48 6,10,8 2D GGILK ± A GLC8 P GLC8_YEAST Protein GLC8 14 CO GGIRE - - RPLA0 P05317 RLA0_YEAST 60S acidic ribosomal protein P0 3,10 2D GIIDK - - YGR1 73W P53295 YG3Y_YEAST Uncharacterized GTP-binding protein YGR173W 14 CO GINNP -(+N.D.) - LSB3 P43603 (*) LSB3_YEAST LAS seventeen-binding protein 3 14 CO GINNP -(+N.D.) - YSC84 P32793 (*) YSC84_YEAST Protein YSC84 14 CO GIPKF - - KEM1 P22147 XRN1_YEAST 5'-3' exoribonuclease 1 14 CO GISRD - - RPS8A P05754 RS8_YEAST 40S ribosomal protein S8 1 MS GITVI - - RBK1 P25332 RBSK_YEAST Probable ribokinase 6,8 2D GKAKK - - FCF1 Q05498 FCF1_YEAST rrna-processing protein FCF1 14 CO GKGKP - - RPS23A P32827 RS23_YEAST 40S ribosomal protein S23 14 CO GKGTP - - RPL37A P49166 (*) RL37A_YEAST 60S ribosomal protein L37-A 14 CO GKGTP - - RPL37B P (*) RL37B_YEAST 60S ribosomal protein L37-B 14 CO GKKNT - - TIF1 1 P38912 IF1A_YEAST Eukaryotic translation initiation factor 1A 14 CO GKNVL - - LYS9 P38999 LYS9_YEAST Saccharopine dehydrogenase [NADP+, L-glutamate-forming] 14,3,6,1 0,8 2D, CO GKSHG - - RPL21A Q02753 RL21A_YEAST 60S ribosomal protein L21-A 1 MS GLFDK - - DDR48 P18899 DDR48_YEAST Stress protein DDR48 14 CO GLTTK - - GPP2 P40106 GPP2_YEAST (DL)-glycerol-3-phosphatase 2 6,8 2D GRMHS - - RPS13 P05756 RS13_YEAST 40S ribosomal protein S13 1 MS GRVIR - - RPL2A P05736 RL2_YEAST 60S ribosomal protein L2 1 MS GSEED - - NOP6 Q07623 NOP6_YEAST Nucleolar protein 6 14 CO GSRRY + A PRE9 P23638 PSA4_YEAST Proteasome component Y13 6,2,8 2D GSRSE + A CKB2 P38930 CSK2C_YEAST Casein kinase II subunit beta' 7 α GTEDA - - HSD1 Q12382 HSD1_YEAST Protein HSD1 14 CO GVEQI(*) - - FBA1 P14540 ALF_YEAST Fructose-bisphosphate aldolase 14,3,6,10,8 2D, CO IIEPS - - UBI2 P14796 RL40_YEAST 60S ribosomal protein L40 14 CO LMPKE(*) -(+N.D.) - RPS10A Q08745 RS10A_YEAST 40S ribosomal protein S10-A 14 CO MAIKK - - NTR2 P36118 NTR2_YEAST Pre-mRNA-splicing factor NTR2 14 CO MAPGK(*) +(-N.D.) N.D. RPL8A P17076 RL8A_YEAST 60S ribosomal protein L8-A 14 CO MDAGL +(-N.D.) B YMR124W P39523 YM11_YEAST Uncharacterized protein YMR124W 14 CO MDAYS +(-N.D.) B FAS1 P07149 FAS1_YEAST Fatty acid synthase subunit beta 14 CO MDEVL +(-N.D.) B COG1 P53079 COG1_YEAST Conserved oligomeric Golgi complex subunit 1 14 CO MDFET +(-N.D.) B SEC23 P15303 SEC23_YEAST Protein transport protein SEC23 14 CO MDGMF +(-N.D.) B TOM6 P33448 TOM6_YEAST Mitochondrial import receptor subunit TOM6 14 CO MDIIL + B PRE1 P22141 PSB2_YEAST Proteasome component C11 14,2 2D, CO MDKKK +(-N.D.) B ARE2 P53629 ARE2_YEAST Sterol O-acyltransferase 2 14 CO MDKNS +(-N.D.) B SDS22 P36047 SDS22_YEAST Protein phosphatase 1 regulatory subunit SDS22 14 CO MDNEV + B SUP45 P12385 ERF1_YEAST Eukaryotic peptide chain release factor subunit 1 6,8 MS MDNGT +(-N.D.) B SRP1 Q02821 IMA1_YEAST Importin subunit alpha 14 CO MDNKT + B RPS28A Q3E7X9 RS28A_YEAST 40S ribosomal protein S28-A 14,1 MS, CO MDNQQ + B IDS2 P46958 IDS2_YEAST IME2-dependent-signaling protein 14 CO MDPEL +(-N.D.) B YMR074C Q04773 YMW4_YEAST Uncharacterized protein YMR074C 14 CO MDPFL +(-N.D.) B RAD28 Q12021 RAD28_YEAST Radiation-sensitive protein CO MDQSV +(-N.D.) B FCF2 Q12035 FCF2_YEAST rrna-processing protein FCF2 14 CO MDSEV + B ACT1 P60010 ACT_YEAST Actin 14,3 2D, CO MDSKT +(-N.D.) B RPS28B P0C0X0 RS28B_YEAST 40S ribosomal protein S28-B 14 CO MDSQP +(-N.D.) B GLC7 P32598 PP12_YEAST Serine/threonine-protein phosphatase PP CO MDSQV +(-N.D.) B TBF1 Q02457 TBF1_YEAST Protein TBF1 14 CO MDTLF + B PAC10 P48363 PFD3_YEAST Prefoldin subunit 3 14 CO MDTVG +(-N.D.) B YDR186C Q04007 YDR86_YEAST Uncharacterized protein YDR186C 14 CO MDTVN +(-N.D.) B ARG82 P07250 IPMK_YEAST Inositol polyphosphate multikinase 14 CO MEAHN + B RNR4 P49723 RIR4_YEAST Ribonucleoside-diphosphate reductase small chain 2 3,6,8 MS, 2D MEFVA(*) +(-N.D.) B TPK2 P06245 KAPB_YEAST camp-dependent protein kinase type 2 14 CO MEHRY + B POM152 P39685 PO152_YEAST Nucleoporin POM152 7 α MELLP +(-N.D.) B GIM3 P53900 PFD4_YEAST Prefoldin subunit 4 14 CO MELSP +(-N.D.) B NUP84 P52891 NUP84_YEAST Nucleoporin NUP84 14 CO MENDK +(-N.D.) B RPS21A P0C0V8 (*) RS21A_YEAST 40S ribosomal protein S21-A 14 CO MENDK +(-N.D.) B RPS21B Q3E754 (*) RS21B_YEAST 40S ribosomal protein S21-B 14 CO MENIT +(-N.D.) B RAD53 P22216 RAD53_YEAST Serine/threonine-protein kinase RAD53 14 CO MEQNA +(-N.D.) B DUS3 Q06053 DUS3_YEAST trna-dihydrouridine synthase 3 14 CO METID +(-N.D.) B OSH3 P38713 OSH3_YEAST Oxysterol-binding protein homolog 3 14 CO METPL +(-N.D.) B LSM3 P57743 LSM3_YEAST U6 snrna-associated Sm-like protein LSm3 14 CO METQP - - CAR1 P00812 ARGI_YEAST Arginase 6,8 2D MFGLP - - TOM5 P80967 TOM5_YEAST Mitochondrial import receptor subunit TOM5 14 CO MFKKF +(-N.D.) C TMA20 P89886 TMA20_YEAST Translation machinery-associated protein CO MFLTR + C PUP2 P32379 PSA5_YEAST Proteasome component PUP2 2 2D MFNTT - - HAD1 P HAO_YEAST 3-hydroxyanthranilate 3,4-dioxygenase 6,8 2D MFRAG + C IMP2 P46972 IMP2_YEAST Mitochondrial inner membrane protease subunit 2 7 α MFRNN + C PRE5 P40302 PSA1_YEAST Proteasome component PRE5 2 2D MFRSV - - LYS12 P40495 LYS12_YEAST Homoisocitrate dehydrogenase, mitochondrial precursor 6,8 2D